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Familial gastric cancer: detection of a hereditary cause helps to understand its etiology
- Ingrid P Vogelaar†1,
- Rachel S van der Post†2,
- Tanya M Bisseling3,
- J Han JM van Krieken2,
- Marjolijn JL Ligtenberg1, 2 and
- Nicoline Hoogerbrugge1Email author
© Vogelaar et al.; licensee BioMed Central Ltd. 2012
Received: 7 November 2012
Accepted: 6 December 2012
Published: 12 December 2012
Worldwide, gastric cancer is one of the most common forms of cancer, with a high morbidity and mortality. Several environmental factors predispose to the development of gastric cancer, such as Helicobacter pylori infection, diet and smoking. Familial clustering of gastric cancer is seen in 10% of cases, and approximately 3% of gastric cancer cases arise in the setting of hereditary diffuse gastric cancer (HDGC). In families with HDGC, gastric cancer presents at relatively young age. Germline mutations in the CDH1 gene are the major cause of HDGC and are identified in approximately 25-50% of families which fulfill strict criteria. Prophylactic gastrectomy is the only option to prevent gastric cancer in individuals with a CDH1 mutation. However, in the majority of families with multiple cases of gastric cancer no germline genetic abnormality can be identified and therefore preventive measures are not available, except for general lifestyle advice. Future research should focus on identifying new genetic predisposing factors for all types of familial gastric cancer.
KeywordsGastric cancer Genetics Hereditary diffuse gastric cancer CDH1 E-cadherin
With an estimated 900,000 new cases per year (8.6% of all new cancer cases except skin cancer), gastric cancer (GC) is the fourth most common form of cancer worldwide. Even though the incidence is rapidly declining in the western world, it is still the second most common cause of death from cancer, with 740,000 deaths annually and a 5-year survival of 20% . High-risk areas include East Asia (Japan, China and Korea), Eastern Europe, and parts of Central and South America. Incidence rates are low in North Europe, North America and Australia . With a mean age at diagnosis above 60 years, gastric cancer is predominantly a disease of the elderly . Only 6–7% of patients with gastric cancer present before the age of 50, and less than 2% before age 40 [2, 3].
Gastric carcinoma is a heterogeneous disease, which is reflected by the diversity of the various histopathological classification schemes . The most commonly used are those of the WHO  and Laurén . The practical scheme of Laurén divides GC roughly into three main types; the diffuse type, the intestinal type and a rest group composed of mixed and indeterminate type . Intestinal GC shows glandular or tubular components with various degrees of differentiation. Diffuse GC consists of poorly cohesive single cells without gland formation. Often signet ring cells are present; therefore it is also referred to as signet ring cell carcinoma . In North America the distribution of the different subtypes is approximately 50% pure intestinal, 35% pure diffuse and 15% mixed diffuse-intestinal .
Etiology of gastric cancer: environmental factors
Gastric cancer is a multifactorial disease, resulting from a combination of environmental factors and genetic alterations. Environmental factors are mainly involved in the etiology of the intestinal type of GC. The main environmental factor involved is Helicobacter pylori (H.Pylori) infection, which is commonly acquired during childhood and persists unless eradicated . H.Pylori can induce a sequence of gastritis, intestinal metaplasia, dysplasia and eventually gastric cancer . A meta-analysis of 12 studies revealed that infection with H.pylori increases the risk of developing GC about sixfold  and the WHO has classified H.pylori as a class I carcinogen in 1994 [10, 11].
Cigarette smoking is an important behavioral risk factor for the development of GC. A large systematic review of 42 studies showed that the risk of GC is increased by 60% in male and 20% in female smokers compared to never smokers . Smoking also enhances the carcinogenic effect of infection with H.pylori. Another important risk factor for the development of GC is diet. An adequate intake of fruit and vegetables likely reduces the risk for developing GC . Salt intake, on the other hand, is strongly associated with an enhanced risk to develop gastric carcinoma. Therefore, diet adjustments that reduce salt intake, for instance after replacement of salt preservation of food by refrigerators, are important factors in the decrease of the incidence of GC, . Smoked meat and fish, pickled vegetables and chili peppers are also associated with GC in some populations . Alcohol consumption has been studied in several populations, but results have been inconclusive .
The incidence of GC is declining worldwide, which is mainly due to the decline in the incidence of the distal, intestinal type of GC. The incidence of diffuse GC, for which no clear environmental risk factors are known, has not decreased . In young people, in whom carcinomas are more likely to be due to genetic susceptibility, a greater proportion shows the diffuse type, suggesting that especially in this subtype germline genetics play a role .
Etiology of gastric cancer: genetic factors
Familial aggregation of gastric cancer is known to occur in approximately 10% of the patients . Epidemiologic studies have shown that in the general population the risk of gastric cancer in first-degree relatives with any type of gastric cancer is increased 2–3 fold . As yet, however, in the vast majority of these patients the underlying genetic cause remains unknown. The most important GC susceptibility gene is CDH1, which accounts for 1-3% of gastric cancers . Predisposing CDH1 mutations have been encountered in about 30% of strictly selected Hereditary Diffuse Gastric Cancer (HDGC) families [17, 18]. Moreover, CDH1 germline mutations may also occur in approximately 7% of patients diagnosed before 50 years of age with tumors exhibiting either a diffuse or a mixed histology . The recognition, surveillance and treatment of CDH1 mutation carriers are extensively described below.
Gastric cancer in familial intestinal gastric cancer and other hereditary cancer syndromes
Many families with intestinal type GC exhibiting an autosomal dominant inheritance pattern have been documented. However, in such families, disease causing germline mutations for intestinal GC have not been found yet.
An increased risk of developing both diffuse and intestinal type GC has been shown in several well known hereditary cancer syndromes, besides HDGC. These syndromes include Lynch syndrome [20–22], Peutz-Jeghers syndrome , Li-Fraumeni syndrome [24–26], hereditary breast and ovarian cancer [27, 28], familial adenomatous polyposis (FAP) [29–31], MUTYH-associated adenomatous polyposis (MAP) , juvenile polyposis syndrome , and Cowden syndrome . The lifetime risk of GC in these syndromes varies substantially between populations studied, but is generally low. For example, although benign gastric abnormalities such as fundic gland polyps develop in approximately 12.5-84% of FAP patients, only 40% of these polyps exhibit adenomatous features and an even smaller percentage (around 0.5%) develops into gastric adenocarcinoma . In Lynch syndrome, the lifetime risk of GC varies between 2.1% in the Netherlands to 30% in Korea . Clearly, the risk of developing GC in these syndromes is higher in areas with high incidence of GC in the general population, such as East Asia, indicating that in these types of hereditary forms of GC environmental factors may play a substantial role. Thus, in all these families life style advice is important, although its effect on GC risk is not precisely known. In most of the mentioned syndromes no consensus exists about recommendations of surveillance of the stomach.
Identification of new genes underlying hereditary gastric cancer
In approximately two thirds of families fulfilling the strict HDGC criteria, no CDH1 mutation is found and they remain genetically unexplained. Most of these families might carry mutations in other, still to be identified, GC susceptibility genes. As binding partner for E-cadherin, mutated β- and γ-catenin have been considered as candidates for diffuse GC predisposition . The β-catenin gene (CTNNB1) was recently assessed in a series of 40 families with positive history of GC from the Netherlands without finding any mutations [Vogelaar et al., unpublished data, 2012].
Also in families with intestinal type GC exhibiting an autosomal dominant inheritance pattern, genetic susceptibility genes may play a role. No gene has been associated with this type of GC yet. In carefully selected patients next generation sequencing based techniques that allow for exome or even genome wide detection of genetic aberrations, might be exploited to unravel genetic predisposition in an unbiased way.
Hereditary diffuse gastric cancer caused by germline CDH1 mutations
In 1998, Guilford et al. identified germline mutations in the CDH1 gene as a cause of hereditary diffuse gastric cancer (HDGC) . CDH1 encodes the protein E-cadherin, which plays an important role in cell–cell adhesion and the maintenance of epithelial integrity . The mutation detection rate is approximately 50% in families with two gastric cancers in first-degree relatives with at least one diffuse gastric cancer (DGC) diagnosed before age 50, or three or more DGC in close relatives diagnosed at any age . The percentage decreases if also single cases of DGC below the age of 35 are included . Germline CDH1 mutations are found in all ethnic groups . The most common types of mutation are small insertions or deletions (35% of the mutations). Missense mutations occur in 28% of families, nonsense mutations and splice site mutations are both observed in 16% of families. Large exonic deletions are relatively rare, with a frequency of about 5% .
For both men and women, CDH1 mutation carriers have a cumulative risk of gastric carcinoma by 80 years of age of 80%, with a mean age at diagnosis of 40 years. Additionally, women carrying a CDH1 mutation have a 60% lifetime risk for developing lobular breast cancer .
Genetic counseling and criteria for CDH1 mutation testing
Clinical criteria for testing for CDH1 germline mutations
1 diffuse gastric cancer case below age 40, or
2 gastric cancer cases in a family, one confirmed diffuse gastric cancer below age 50, or
3 confirmed diffuse gastric cancer cases in 1st or 2nd degree relatives independent of age, or
Personal or family history of diffuse gastric cancer and lobular breast cancer, with one diagnosis below age 50
Proposed mechanism of HDGC initiation
In 2009, Humar and Guilford proposed a mechanism of HDGC initiation . E-cadherin is known to play an important role in cell polarity and epithelial tissue architecture [45, 46]. It is proposed that mutations in CDH1 disturb the cell-cell adhesion mediated by E-cadherin, which causes disruption of the correct spatial organization of the cells. This in turn may interfere with processes that regulate cell division, such as the orientation of the mitotic spindle. Abrogated cell polarity may also lead to the disruption of cell fate determination [44, 47, 48]. These disturbed processes can ultimately result in the displacement of cells with self-renewal capacity into the lamina propria and lead to the formation of signet ring cell carcinomas with the capacity for sustained cell division and thus to progression .
Prophylactic total gastrectomy in CDH1 mutation carriers
Prophylactic gastrectomy is currently the only option to eliminate risk of GC development in CDH1 mutation carriers . The prognosis of patients with a prophylactic gastrectomy is very good. The estimated overall mortality for total gastrectomy is 2–4% with a nearly 100% risk of long-term morbidity. Associated problems following gastrectomy include abdominal pain after eating, dumping syndrome, lactose intolerance, fat malabsorption and steatorrhoea and postprandial fullness [40, 50–52]. The optimal timing of prophylactic gastrectomy in individuals with CDH1 mutations is not yet known. Preventive gastrectomy specimens of CDH1 mutation carriers reveal multiple small signet ring cell lesions with low proliferation rates; few of these lesions progress to an aggressive carcinoma beyond the muscular mucosa . It is unknown why only some of these lesions develop into aggressive carcinomas. No correlation between patient age and number of small signet ring cell foci has been observed. Blair et al. advise CDH1 mutation carriers with normal gastric biopsies to consider gastrectomy once the individuals are older than 20 years of age . Other authors recommend considering preventive gastrectomy when the CDH1 mutation carrier is 5 years younger than the youngest family member with DGC, which generally means that preventive gastrectomy is postponed to an age later than 18 years .
In case of a preventive gastrectomy, total gastrectomy with Roux-en-Y reconstruction is recommended. There is no need for a radical lymph node dissection in the prophylactic setting since mucosal adenocarcinomas without submucosal invasion have a low risk of lymph node metastases .
Pathological analysis of preventive gastrectomy specimens
Pathological analysis of the entire gastrectomy sample includes a thorough assessment microscopically with haematoxylin and eosin (H&E) and a mucin stain, such as periodic acid-Schiff (PAS). PAS-staining has proven to be helpful as a primary stain, increasing the detection rate of small invasive signet ring cell foci and reducing screening time . The ‘Swiss roll’ technique can be used to include the complete mucosa . The pathology report should mention all gastric abnormalities and localization, as (pre-) malignant lesions, intestinal metaplasia, dysplasia, inflammation and presence of H.pylori-associated gastritis. Histological confirmation of resection margins consisting of proximal esophageal and distal duodenal mucosa is essential, since new GC can develop in remaining gastric mucosa.
Pathology of HDGC
The ‘Cambridge surveillance protocol’ is advised for CDH1 mutation carriers who do not (yet) want to undergo a prophylactic gastrectomy, to individuals at 50% risk of being carrier who are not (yet) willing to be tested for the mutation as well as for members from HDGC families without a known CDH1 mutation . This protocol comprises H.Pylori-testing, annual gastroscopy with ‘high definition’ endoscope, careful inspection of mucosa during 30 minutes, insufflation and desufflation of the stomach, biopsies of mucosal abnormalities and 30 random biopsies from different gastric regions (antrum, angulus, corpus, fundus, cardia) . The endoscopy should be performed using a white light high definition endoscope in a dedicated session with at least 30 minutes allocated to allow for a careful inspection of the mucosa on inflation and deflation, and to allow time for multiple biopsies to be taken . Use of mucolytics such as acetylcysteine may be helpful to obtain good views. Endoscopy permits direct inspection and biopsy of suspicious areas, but diffuse GC is difficult to detect at an early and treatable stage since the lesions tend to spread into the lamina propria without visible exophytic masses. The major problems include difficulties to identify (sub)mucosal lesions and biases in sampling in macroscopically normal-appearing gastric mucosa . Such specimens therefore need to be evaluated by pathologists with expertise with this type of lesions. Several studies have shown that even though CDH1 mutation carriers had negative biopsies prior to prophylactic gastrectomy, foci were detected in their gastrectomy specimens [49, 53, 58, 59]. Other techniques, such as chromoendoscopic techniques, trimodal imaging, confocal endomicroscopy and molecular imaging techniques are currently not recommended, but need to be further explored in a research setting .
The overall incidence of GC is declining, which is most likely due to the reduction in environmental risk factors. Germline mutations in the CDH1 gene have been identified as an important cause of HDGC, but still in more than two thirds of strictly selected HDGC families the genetic cause remains unknown. Additionally, the genetic basis of familial cases with an intestinal type gastric cancer is largely unknown. Elucidation of novel gastric cancer susceptibility genes will be an important step towards additional options for gastric cancer prevention. Therefore, identifying new genetic gastric cancer predisposing factors is one of the important targets in the near future.
- Parkin DM, Bray F, Ferlay J, Pisani P: Global cancer statistics, 2002. CA Cancer J Clin 2005, 55: 74–108. 10.3322/canjclin.55.2.74View ArticlePubMedGoogle Scholar
- Curado MP, Edwards B, Shin HR, Storm H, Ferlay J, Heanue M, Boyle P: Cancer incidence in Five Continents. Lyon, France: IARC Scientific publication; 2007.Google Scholar
- Yamaoka Y, Kato M, Asaka M: Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med 2008, 47: 1077–1083. 10.2169/internalmedicine.47.0975View ArticlePubMedPubMed CentralGoogle Scholar
- Bosman FT, Carneiro F, Hruban RH, Theise ND: WHO Classification of Tumours of the Digestive System. 4th edition. Lyon, France: IARC; 2010.Google Scholar
- Lauren P: The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma. An attempt at a histo-clinical classification. Acta Pathol Microbiol Scand 1965, 64: 31–49.PubMedGoogle Scholar
- Pisani P, Bray F, Parkin DM: Estimates of the world-wide prevalence of cancer for 25 sites in the adult population. Int J Cancer 2002, 97: 72–81. 10.1002/ijc.1571View ArticlePubMedGoogle Scholar
- Bouvard V, Baan R, Straif K, Grosse Y, Secretan B, El-Ghissassi F, Benbrahim-Tallaa L, Guha N, Freeman C, Galichet L, Cogliano V: A review of human carcinogens--Part B: biological agents. Lancet Oncol 2009, 10: 321–322. 10.1016/S1470-2045(09)70096-8View ArticlePubMedGoogle Scholar
- Correa P: Human gastric carcinogenesis: a multistep and multifactorial process–First American Cancer Society Award Lecture on Cancer Epidemiology and Prevention. Cancer Res 1992, 52: 6735–6740.PubMedGoogle Scholar
- Helicobacter and Cancer Collaborative Group: Gastric cancer and Helicobacter pylori: a combined analysis of 12 case control studies nested within prospective cohorts. Gut 2001, 49: 347–353. 10.1136/gut.49.3.347View ArticleGoogle Scholar
- no authors listed: Infection with Helicobacter pylori. IARC Monogr Eval Carcinog Risks Hum 1994, 61: 177–240.Google Scholar
- Suerbaum S, Michetti P: Helicobacter pylori infection. N Engl J Med 2002, 347: 1175–1186. 10.1056/NEJMra020542View ArticlePubMedGoogle Scholar
- Ladeiras-Lopes R, Pereira AK, Nogueira A, Pinheiro-Torres T, Pinto I, Santos-Pereira R, Lunet N: Smoking and gastric cancer: systematic review and meta-analysis of cohort studies. Cancer Causes Control 2008, 19: 689–701. 10.1007/s10552-008-9132-yView ArticlePubMedGoogle Scholar
- Gonzalez CA, Lopez-Carrillo L: Helicobacter pylori, nutrition and smoking interactions: their impact in gastric carcinogenesis. Scand J Gastroenterol 2010, 45: 6–14. 10.3109/00365520903401959View ArticlePubMedGoogle Scholar
- La Vecchia C, Negri E, Franceschi S, Gentile A: Family history and the risk of stomach and colorectal cancer. Cancer 1992, 70: 50–55. 10.1002/1097-0142(19920701)70:1<50::AID-CNCR2820700109>3.0.CO;2-IView ArticlePubMedGoogle Scholar
- Goldgar DE, Easton DF, Cannon-Albright LA, Skolnick MH: Systematic population-based assessment of cancer risk in first-degree relatives of cancer probands. J Natl Cancer Inst 1994, 86: 1600–1608. 10.1093/jnci/86.21.1600View ArticlePubMedGoogle Scholar
- Stone J, Bevan S, Cunningham D, Hill A, Rahman N, Peto J, Marossy A, Houlston RS: Low frequency of germline E-cadherin mutations in familial and nonfamilial gastric cancer. Br J Cancer 1999, 79: 1935–1937. 10.1038/sj.bjc.6690308View ArticlePubMedPubMed CentralGoogle Scholar
- Kaurah P, MacMillan A, Boyd N, Senz J, De LA, Chun N, Suriano G, Zaor S, Van ML, Gilpin C, et al.: Founder and recurrent CDH1 mutations in families with hereditary diffuse gastric cancer. JAMA 2007, 297: 2360–2372. 10.1001/jama.297.21.2360View ArticlePubMedGoogle Scholar
- Oliveira C, Senz J, Kaurah P, Pinheiro H, Sanges R, Haegert A, Corso G, Schouten J, Fitzgerald R, Vogelsang H, et al.: Germline CDH1 deletions in hereditary diffuse gastric cancer families. Hum Mol Genet 2009, 18: 1545–1555. 10.1093/hmg/ddp046View ArticlePubMedPubMed CentralGoogle Scholar
- Corso G, Pedrazzani C, Pinheiro H, Fernandes E, Marrelli D, Rinnovati A, Pascale V, Seruca R, Oliveira C, Roviello F: E-cadherin genetic screening and clinico-pathologic characteristics of early onset gastric cancer. Eur J Cancer 2011, 47: 631–639. 10.1016/j.ejca.2010.10.011View ArticlePubMedGoogle Scholar
- Park YJ, Shin KH, Park JG: Risk of gastric cancer in hereditary nonpolyposis colorectal cancer in Korea. Clin Cancer Res 2000, 6: 2994–2998.PubMedGoogle Scholar
- Capelle LG, Van Grieken NC, Lingsma HF, Steyerberg EW, Klokman WJ, Bruno MJ, Vasen HF, Kuipers EJ: Risk and epidemiological time trends of gastric cancer in Lynch syndrome carriers in the Netherlands. Gastroenterology 2010, 138: 487–492. 10.1053/j.gastro.2009.10.051View ArticlePubMedGoogle Scholar
- Sereno M, Aguayo C, Guillen PC, Gomez-Raposo C, Zambrana F, Gomez-Lopez M, Casado E: Gastric tumours in hereditary cancer syndromes: clinical features, molecular biology and strategies for prevention. Clin Transl Oncol 2011, 13: 599–610. 10.1007/s12094-011-0705-yView ArticlePubMedGoogle Scholar
- van Lier MG, Westerman AM, Wagner A, Looman CW, Wilson JH, de Rooij FW, Lemmens VE, Kuipers EJ, Mathus-Vliegen EM, van Leerdam ME: High cancer risk and increased mortality in patients with Peutz-Jeghers syndrome. Gut 2011, 60: 141–147. 10.1136/gut.2010.223750View ArticlePubMedGoogle Scholar
- Oliveira C, Ferreira P, Nabais S, Campos L, Ferreira A, Cirnes L, Alves CC, Veiga I, Fragoso M, Regateiro F, et al.: E-Cadherin (CDH1) and p53 rather than SMAD4 and Caspase-10 germline mutations contribute to genetic predisposition in Portuguese gastric cancer patients. Eur J Cancer 2004, 40: 1897–1903. 10.1016/j.ejca.2004.04.027View ArticlePubMedGoogle Scholar
- Keller G, Vogelsang H, Becker I, Plaschke S, Ott K, Suriano G, Mateus AR, Seruca R, Biedermann K, Huntsman D, et al.: Germline mutations of the E-cadherin(CDH1) and TP53 genes, rather than of RUNX3 and HPP1, contribute to genetic predisposition in German gastric cancer patients. J Med Genet 2004, 41: e89. 10.1136/jmg.2003.015594View ArticlePubMedPubMed CentralGoogle Scholar
- Masciari S, Dewanwala A, Stoffel EM, Lauwers GY, Zheng H, Achatz MI, Riegert-Johnson D, Foretova L, Silva EM, Digianni L, et al.: Gastric cancer in individuals with Li-Fraumeni syndrome. Genet Med 2011, 13: 651–657. 10.1097/GIM.0b013e31821628b6View ArticlePubMedPubMed CentralGoogle Scholar
- Jakubowska A, Nej K, Huzarski T, Scott RJ, Lubinski J: BRCA2 gene mutations in families with aggregations of breast and stomach cancers. Br J Cancer 2002, 87: 888–891. 10.1038/sj.bjc.6600562View ArticlePubMedPubMed CentralGoogle Scholar
- Friedenson B: BRCA1 and BRCA2 pathways and the risk of cancers other than breast or ovarian. MedGenMed 2005, 7: 60.PubMedPubMed CentralGoogle Scholar
- Iwama T, Mishima Y, Utsunomiya J: The impact of familial adenomatous polyposis on the tumorigenesis and mortality at the several organs. Its rational treatment. Ann Surg 1993, 217: 101–108. 10.1097/00000658-199302000-00002View ArticlePubMedPubMed CentralGoogle Scholar
- Park SY, Ryu JK, Park JH, Yoon H, Kim JY, Yoon YB, Park JG, Lee SH, Kang SB, Park JW, Oh JH: Prevalence of gastric and duodenal polyps and risk factors for duodenal neoplasm in korean patients with familial adenomatous polyposis. Gut Liver 2011, 5: 46–51. 10.5009/gnl.2011.5.1.46View ArticlePubMedPubMed CentralGoogle Scholar
- Lynch HT, Snyder C, Davies JM, Lanspa S, Lynch J, Gatalica Z, Graeve V, Foster J: FAP, gastric cancer, and genetic counseling featuring children and young adults: a family study and review. Fam Cancer 2010, 9: 581–588. 10.1007/s10689-010-9352-7View ArticlePubMedGoogle Scholar
- Win AK, Hopper JL, Jenkins MA: Association between monoallelic MUTYH mutation and colorectal cancer risk: a meta-regression analysis. Fam Cancer 2011, 10: 1–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Pollock J, Welsh JS: Clinical cancer genetics: Part I: Gastrointestinal. Am J Clin Oncol 2011, 34: 332–336. 10.1097/COC.0b013e3181dea432View ArticlePubMedGoogle Scholar
- Stanich PP, Francis DL, Sweetser S: The spectrum of findings in Cowden syndrome. Clin Gastroenterol Hepatol 2011, 9: e2-e3. 10.1016/j.cgh.2010.07.003View ArticlePubMedGoogle Scholar
- Abraham SC, Nobukawa B, Giardiello FM, Hamilton SR, Wu TT: Fundic gland polyps in familial adenomatous polyposis: neoplasms with frequent somatic adenomatous polyposis coli gene alterations. Am J Pathol 2000, 157: 747–754. 10.1016/S0002-9440(10)64588-9View ArticlePubMedPubMed CentralGoogle Scholar
- Koornstra JJ, Mourits MJ, Sijmons RH, Leliveld AM, Hollema H, Kleibeuker JH: Management of extracolonic tumours in patients with Lynch syndrome. Lancet Oncol 2009, 10: 400–408. 10.1016/S1470-2045(09)70041-5View ArticlePubMedGoogle Scholar
- Lynch HT, Grady W, Suriano G, Huntsman D: Gastric cancer: new genetic developments. J Surg Oncol 2005, 90: 114–133. 10.1002/jso.20214View ArticlePubMedGoogle Scholar
- Guilford P, Hopkins J, Harraway J, McLeod M, McLeod N, Harawira P, Taite H, Scoular R, Miller A, Reeve AE: E-cadherin germline mutations in familial gastric cancer. Nature 1998, 392: 402–405. 10.1038/32918View ArticlePubMedGoogle Scholar
- Berx G, Becker KF, Hofler H, van RF: Mutations of the human E-cadherin (CDH1) gene. Hum Mutat 1998, 12: 226–237. 10.1002/(SICI)1098-1004(1998)12:4<226::AID-HUMU2>3.0.CO;2-DView ArticlePubMedGoogle Scholar
- Fitzgerald RC, Hardwick R, Huntsman D, Carneiro F, Guilford P, Blair V, Chung DC, Norton J, Ragunath K, van Krieken JH, et al.: Hereditary diffuse gastric cancer: updated consensus guidelines for clinical management and directions for future research. J Med Genet 2010, 47: 436–444. 10.1136/jmg.2009.074237View ArticlePubMedPubMed CentralGoogle Scholar
- Carneiro F, Oliveira C, Suriano G, Seruca R: Molecular pathology of familial gastric cancer, with an emphasis on hereditary diffuse gastric cancer. J Clin Pathol 2008, 61: 25–30.View ArticlePubMedGoogle Scholar
- Pharoah PD, Guilford P, Caldas C: Incidence of gastric cancer and breast cancer in CDH1 (E-cadherin) mutation carriers from hereditary diffuse gastric cancer families. Gastroenterology 2001, 121: 1348–1353. 10.1053/gast.2001.29611View ArticlePubMedGoogle Scholar
- Blair V, Martin I, Shaw D, Winship I, Kerr D, Arnold J, Harawira P, McLeod M, Parry S, Charlton A, et al.: Hereditary diffuse gastric cancer: diagnosis and management. Clin Gastroenterol Hepatol 2006, 4: 262–275. 10.1016/j.cgh.2005.12.003View ArticlePubMedGoogle Scholar
- Humar B, Guilford P: Hereditary diffuse gastric cancer: a manifestation of lost cell polarity. Cancer Sci 2009, 100: 1151–1157. 10.1111/j.1349-7006.2009.01163.xView ArticlePubMedGoogle Scholar
- Nejsum LN, Nelson WJ: A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity. J Cell Biol 2007, 178: 323–335. 10.1083/jcb.200705094View ArticlePubMedPubMed CentralGoogle Scholar
- Drubin DG, Nelson WJ: Origins of cell polarity. Cell 1996, 84: 335–344. 10.1016/S0092-8674(00)81278-7View ArticlePubMedGoogle Scholar
- Lechler T, Fuchs E: Asymmetric cell divisions promote stratification and differentiation of mammalian skin. Nature 2005, 437: 275–280. 10.1038/nature03922View ArticlePubMedPubMed CentralGoogle Scholar
- Lu B, Roegiers F, Jan LY, Jan YN: Adherens junctions inhibit asymmetric division in the Drosophila epithelium. Nature 2001, 409: 522–525. 10.1038/35054077View ArticlePubMedGoogle Scholar
- Norton JA, Ham CM, Van DJ, Jeffrey RB, Longacre TA, Huntsman DG, Chun N, Kurian AW, Ford JM: CDH1 truncating mutations in the E-cadherin gene: an indication for total gastrectomy to treat hereditary diffuse gastric cancer. Ann Surg 2007, 245: 873–879. 10.1097/01.sla.0000254370.29893.e4View ArticlePubMedPubMed CentralGoogle Scholar
- Fitzgerald RC, Caldas C: E-cadherin mutations and hereditary gastric cancer: prevention by resection? Dig Dis 2002, 20: 23–31. 10.1159/000063162View ArticlePubMedGoogle Scholar
- Miholic J, Meyer HJ, Muller MJ, Weimann A, Pichlmayr R: Nutritional consequences of total gastrectomy: the relationship between mode of reconstruction, postprandial symptoms, and body composition. Surgery 1990, 108: 488–494.PubMedGoogle Scholar
- Cisco RM, Ford JM, Norton JA: Hereditary diffuse gastric cancer: implications of genetic testing for screening and prophylactic surgery. Cancer 2008, 113: 1850–1856. 10.1002/cncr.23650View ArticlePubMedGoogle Scholar
- Barber ME, Save V, Carneiro F, Dwerryhouse S, Lao-Sirieix P, Hardwick RH, Caldas C, Fitzgerald RC: Histopathological and molecular analysis of gastrectomy specimens from hereditary diffuse gastric cancer patients has implications for endoscopic surveillance of individuals at risk. J Pathol 2008, 216: 286–294. 10.1002/path.2415View ArticlePubMedGoogle Scholar
- Charlton A, Blair V, Shaw D, Parry S, Guilford P, Martin IG: Hereditary diffuse gastric cancer: predominance of multiple foci of signet ring cell carcinoma in distal stomach and transitional zone. Gut 2004, 53: 814–820. 10.1136/gut.2002.010447View ArticlePubMedPubMed CentralGoogle Scholar
- Sano T, Kobori O, Muto T: Lymph node metastasis from early gastric cancer: endoscopic resection of tumour. Br J Surg 1992, 79: 241–244. 10.1002/bjs.1800790319View ArticlePubMedGoogle Scholar
- Lee AF, Rees H, Owen DA, Huntsman DG: Periodic acid-schiff is superior to hematoxylin and eosin for screening prophylactic gastrectomies from CDH1 mutation carriers. Am J Surg Pathol 2010, 34: 1007–1013. 10.1097/PAS.0b013e3181e28985View ArticlePubMedGoogle Scholar
- Park CM, Reid PE, Walker DC, MacPherson BR: A simple, practical 'swiss roll' method of preparing tissues for paraffin or methacrylate embedding. J Microsc 1987, 145: 115–120. 10.1111/j.1365-2818.1987.tb01321.xView ArticlePubMedGoogle Scholar
- Chun YS, Lindor NM, Smyrk TC, Petersen BT, Burgart LJ, Guilford PJ, Donohue JH: Germline E-cadherin gene mutations: is prophylactic total gastrectomy indicated? Cancer 2001, 92: 181–187. 10.1002/1097-0142(20010701)92:1<181::AID-CNCR1307>3.0.CO;2-JView ArticlePubMedGoogle Scholar
- Huntsman DG, Carneiro F, Lewis FR, MacLeod PM, Hayashi A, Monaghan KG, Maung R, Seruca R, Jackson CE, Caldas C: Early gastric cancer in young, asymptomatic carriers of germ-line E-cadherin mutations. N Engl J Med 2001, 344: 1904–1909. 10.1056/NEJM200106213442504View ArticlePubMedGoogle Scholar
- Carneiro F, Huntsman DG, Smyrk TC, Owen DA, Seruca R, Pharoah P, Caldas C, Sobrinho-Simoes M: Model of the early development of diffuse gastric cancer in E-cadherin mutation carriers and its implications for patient screening. J Pathol 2004, 203: 681–687. 10.1002/path.1564View ArticlePubMedGoogle Scholar
- Oliveira C, Seruca R, Carneiro F: Genetics, pathology, and clinics of familial gastric cancer. Int J Surg Pathol 2006, 14: 21–33. 10.1177/106689690601400105View ArticlePubMedGoogle Scholar
- Kluijt I, Sijmons RH, Hoogerbrugge N, Plukker JT, De JD, van Krieken JH, Van HR, Ligtenberg M, Bleiker E, Cats A: Familial gastric cancer: guidelines for diagnosis, treatment and periodic surveillance. Fam Cancer 2012, 11: 363–369. 10.1007/s10689-012-9521-yView ArticlePubMedGoogle Scholar
- Fitzgerald RC, Caldas C: Clinical implications of E-cadherin associated hereditary diffuse gastric cancer. Gut 2004, 53: 775–778. 10.1136/gut.2003.022061View ArticlePubMedPubMed CentralGoogle Scholar
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