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Molecular study of the perforin gene in familial hematological malignancies
- Rim El Abed1,
- Violaine Bourdon2,
- Ilia Voskoboinik3,
- Halima Omri4,
- Yosra Ben Youssef1, 4,
- Mohamed Adnene Laatiri4,
- Laetitia Huiart2,
- François Eisinger2,
- Laetitia Rabayrol2,
- Marc Frenay5,
- Paul Gesta6,
- Liliane Demange7,
- Hélène Dreyfus8,
- Valérie Bonadona9,
- Catherine Dugast10,
- Hélène Zattara11,
- Laurence Faivre12, 13,
- Monia Zaier1, 4,
- Saloua Yacoub Jemni14,
- Testsuro Noguchi2,
- Hagay Sobol2, 15Email author and
- Zohra Soua1
© El Abed et al; licensee BioMed Central Ltd. 2011
Received: 3 December 2010
Accepted: 21 September 2011
Published: 21 September 2011
Perforin gene (PRF1) mutations have been identified in some patients diagnosed with the familial form of hemophagocytic lymphohistiocytosis (HLH) and in patients with lymphoma. The aim of the present study was to determine whether patients with a familial aggregation of hematological malignancies harbor germline perforin gene mutations. For this purpose, 81 unrelated families from Tunisia and France with aggregated hematological malignancies were investigated. The variants detected in the PRF1 coding region amounted to 3.7% (3/81). Two of the three variants identified were previously described: the p.Ala91Val pathogenic mutation and the p.Asn252Ser polymorphism. A new p.Ala 211Val missense substitution was identified in two related Tunisian patients. In order to assess the pathogenicity of this new variation, bioinformatic tools were used to predict its effects on the perforin protein structure and at the mRNA level. The segregation of the mutant allele was studied in the family of interest and a control population was screened. The fact that this variant was not found to occur in 200 control chromosomes suggests that it may be pathogenic. However, overexpression of mutated PRF1 in rat basophilic leukemia cells did not affect the lytic function of perforin differently from the wild type protein.
Perforin is a Ca2+ dependent pore forming protein stored as an active protein in specialized secretory lysosomes (known as lytic granules) of Cytotoxic T lymphocyte (CTL) and Natural Killer cells (NK). Upon recognition of the target cells, lytic granules polarize and release their contents at the immunologic synapse, which triggers apoptosis [1, 2]. Cytotoxic granules also contain a group of serine proteases called granzymes in a proteoglycan matrix [3, 4]. Perforin is the only molecule that is able to deliver granzymes into the target cell.
Perforin is encoded by PRF1, a highly conserved gene, which is crucial to the function of the granzymes involved in triggering caspase dependent and caspase independent target cell death after the formation of an immunological synapse . Perforin-mediated cellular cytotoxicity is a highly preserved mechanism responsible for killing virus-infected and neoplastic cells.
PRF1 mutations were first described in familial hemophagocytic lymphohistiocytosis (FHL) [6, 7]. These mutations include nonsense, frameshift and missense mutations disrupting perforin activity [8–15]. FHL is a life threatening disease usually occurring in childhood, which is associated with profound immune derangement and characterized by impaired T-cell and NK cell granule-mediated cytotoxic activity. The fact that these mutations were described in homozygous and compound heterozygous states suggests that autosomal recessive transmission processes are involved. Patients with FHL caused by biallelic perforin mutations are severely immunocompromised [7, 16].
Inherited PRF1 mutations were subsequently described in various types of lymphomas [17–19], which suggests that PRF1 protein is involved in the immune surveillance mechanisms preventing tumor growth and/or development.
The key role of perforin in immune surveillance has been extensively investigated using perforin knockout (PRF1-KO) mice, which show high sensitivity to several viral infections. These PRF1-KO mice develop spontaneous and aggressive disseminated B-cell lymphoma, and fail to efficiently reject many transplanted tumors [24–27].
Nevertheless, some previous studies have shown that hematological neoplasms can be transmitted vertically, which suggests that a predisposition may be caused by an inherited genetic factor with incomplete penetrance and pleitropic effects [28, 29]. In view of these experimental findings, we tested the hypothesis that some patients with familial hematological malignancies might harbor perforin gene mutations. PRF1 germline mutations were therefore analyzed in a panel of families with aggregated hematological malignancies with or without solid tumors.
Patients and control population
The entire PRF1 coding region was sequenced in 89 patients belonging to 81 independent families: 6 Tunisian and 75 French families recruited via a national cooperative network focusing on familial hematological malignancies (call for proposal 2005) and the GenHem-INSERM/DGRS Franco- Tunisian project, and this network was supported by the French National Cancer Institute (INCa). The cohort consisted of 71 patients belonging to 63 familial forms of hematological malignancy (at least two cases of hematological malignancy with or without solid tumors had occurred in the patients' first, second or third degree relatives); 17 patients from 17 families with aggregated tumors where one case of hematological malignancy had occurred in the patients' first, second or third degree relatives; and 1 patient who had a multiple primitive tumor with hematological malignancy but no family history.
The genetic analysis was performed on genomic DNA extracted from peripheral blood cells obtained during complete remission. Informed consent was obtained from the patients, relevant family members (healthy relatives) or their legal guardian as required by the Helsinki Declaration. In the only case where no peripheral blood was available, tumoral DNA was prepared from paraffin embedded sections as previously described [30, 31].
A control Tunisian population was recruited among healthy blood donors. Blood samples were obtained after the donors had given their informed consent.
Analysis of perforin gene mutations
Genomic DNA was extracted from whole blood with the EZ1 DNA tissue kit (Qiagen, Hilden, Germany) in line with the manufacturer's instructions. The coding region (exon 2 and 3) and the intron-exon junctions of the perforin were amplified using standard PCR methods. The primer sequences are available upon request.
The amplified PCR products were column-purified and both strands were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit v1.1 (Applied Biosystems- Foster City, USA) and loaded onto an ABI Prism 3130 sequencer (Applied biosystems). The sequence chromatograms obtained were compared with the published human PRF1 gene sequence (Genebank accession number M28393) using the SeqScape software program v2.5 (Applied Biosystems).
To address the issue of the occurrence of the newly observed PRF1 mutation c.632 C > T (p.Ala211Val) in the general population, a series of 200 Tunisian control chromosomes were examined.
In silico analysis
To predict the effects of non synonymous SNP (nsSNP) at mRNA and amino acid levels, Align-GVGD, SIFT, ESEfinder and ESErescue bioinformatic tools, provided in the Alamut pack V2.0 http://www.interactive-biosoftware.com. Align-GVGD is a program that uses the biophysical characteristics of amino acids combined with protein multiple sequence alignments (Grantham variation and Grantham Deviation scores) to predict where amino acid substitutions fall in a spectrum ranging from enriched deleterious to enriched neutral, based on the GV an GD scores (0 to > 200). The criteria were set at GV = 0 and GD≥65, respectively . The SIFT (Sorting Intolerant from Tolerant) method predicts whether an amino acid substitution affects protein function based on sequence homology and the physical properties of amino acids. Normalized probabilities of substitutions are calculated under default settings and probabilities ≤0.05 are taken to be deleterious . ESEfinder [34, 35] and ESErescue  are algorithms for analyzing several potential splicing regulatory elements.
Cell cultures, cytotoxicity assays and perforin expression
The p.Ala211Val-PRF1 variant was generated using the QuickChange Protocol (Stratagene) (forward primer -5' CCAGCCCGTCTACCTCAGGC-, reverse primer -5' GCCTGAGGTAGACGGGCTGG-). The wild-type (WT) and mutant cDNA were cloned into the pIRES2-EGFP (CLONTECH) expression vector. Effector rat basophile leukaemia cells (RBL-2H3; ATCC) were cultured and transiently transfected with either wild-type or p.Ala211Val mutant variant, and populations of cells with identical mean EGFP fluorescence were FACS sorted as previously described . The resultant cells were then surface labeled with anti-trinitrophenol IgE; target Jurkat T cells were first loaded with 51 Cr and then surface-labeled with trinitrobenzosulfonic acid, as previously described . RBL and Jurkat T cells were subsequently mixed at various effector/target cell ratios, and the supernatants were harvested after a 4-hour period of incubation at 37°C. The 51 Cr released was measured on a gamma counter. The percentage rate of specific 51 Cr release was calculated using the formula [(experimental value-spontaneous release)/(total value-spontaneous release) ×100%]. This mutated perforin was tested 3 times at E/T ratios of 30:1, 10:1 and 2:1.
Summary of results of PRF1 mutation screening on a cohort of 89 patients
Age at diagnosis
Amino acid substitution
c.632 C > T
FHM without solid tumors
membrane attack complex component/perforin/complement C9
c.632 C > T
FHM without solid tumors
membrane attack complex component/perforin/complement C9
c.755 A > G
FHM with solid tumors
membrane attack complex component/perforin/complement C9
renal cancer, Ichthyosis
c.272 C > T
FHM with solid tumors
? Low homology
This proband had a female second cousin, who was diagnosed at the age of 36 (in 1998) with HL stage IV B b. She carried the same heterozygous missense mutation (p.Ala211Val). The treatment failed, and she relapsed twice, two and three years later, and died due to the progression of the disease. Neither her sisters nor her brother carry the mutation. Her parents' marriage was consanguineous (Figure 1).
The Align-GVGD and SIFT scores obtained on the p. Ala211Val substitution (0.35 and Class C0 (GV: 353.86, GD = 0), respectively) predicted no effect on the protein structure. The Alanine residue at position 211 was not a highly conserved amino acid (6/17 species Alamut V2.0) and the Grantham score between Alanine and Valine was 64, which is a relatively low score.
In addition, the 632C > T substitution is found to be localized 100 bp downstream of the consensus splice acceptor site. Changes in the exonic splicing enhancer pattern were observed only with ESE finder (the creation of a SRp55 motif scored 3.01 using ESEfinder). No new hexamers were predicted in this position by ESErescue. The in silico analysis of c.632 C > T therefore did not predict that this substitution was deleterious.
In parallel, in order to investigate the occurrence of the p.Ala211Val mutation in the population, 200 Tunisian control chromosomes were screened for the PRF1 gene. Interestingly, the p.Ala211Val substitution was not detected in any of the control cases.
The patient from family F2, a French male diagnosed at the age of 45 years with a non Hodgkin lymphoma (NHL), showed a heterozygous mutation c.755 G > A in exon 3 resulting in Asparagine being replaced by Serine in position 252 (p.Asn252Ser). This patient had a twin brother who died at the age of 2 from leukemia. The familial anamnesis showed signs of gastric tumors developed by the father and the grandfather at the age of 54 and 50, respectively.
The patient from French family F3, a female diagnosed at 43 years with renal cancer and ichtyosis, had a heterozygous mutation c.272 C > T in exon 2, where the Alanine in position 91 had been transformed into Valine (p.Ala91Val). Her family had suffered from multiple occurrences of cancer, as a brother and a cousin both developed chronic myeloid leukemia (CML) at the age of 34 and 37 years, respectively. The brother died of the disease.
The rejection of many experimental cancers by CTLs and NK cells is dependent on the pore forming protein perforin. This protein plays an important role in maintaining immune surveillance, which protects the organism from the development of lymphoproliferative disorders in vivo. Therefore, mice lacking peforin are profoundly immunodeficient and have an enhanced susceptibility to viral infection and cancer. In these experimental animal models, the increased incidence of lymphoma associated with perforin deficiency is conceivably due to a defective cytotoxic mechanism normally involved in maintaining immune homeostasis: this may favor the uncontrolled proliferation and development of premalignant lymphoid cells which may thus acquire a malignant phenotype. Stepp et al first suggested that in humans, perforin deficiency is responsible for an acute immune disregulation leading to HLH . Based on mutational analyses, other authors reported the occurrence of lymphomas including B-cell, T-cell non-Hodgkin lymphoma as well as Hodgkin disease and autoimmune lymphoproliferative syndrome (ALPS) that were associated with perforin gene mutations. Bolitho et al recently established that perforin is involved in the surveillance of B cell lymphomas, as opposed to perforin loss being causative of lymphoma .
In the present study, the rate of occurrence of missense mutations in familial hematological malignancies was found to be 3.7%. The p.Ala211Val missense mutation detected in one Tunisian patient is a novel finding in the perforin gene: this is the first time to our knowledge that this variant has been described. The two other substitutions, p.Ala91Val and p.Asn252Ser which were identified in French patients, have been previously described and largely investigated.
The p.Asn252Ser PRF1 variant was first reported in a patient with FHL , and then identified in patient presenting with ALPS and NHL . A functional analysis  and studies on a control population  have suggested that the p.Asn252Ser variant may be a benign polymorphism. p.Asn252Ser has since been proved to be a neutral PRF1 polymorphism.
The p.Ala91Val PRF1 variant has been previously reported in siblings with FHL and NHL [17, 19], and in patients with Dianzani autoimmune lymphoproliferative disease  and aplastic anemia . Here we identified this mutation in a patient with renal cancer with a family history of chronic myeloid leukemia (CML). Functional studies have shown that the levels of expression of the p.Ala91Val PRF1 protein decreased in these patients, resulting in partial loss of lytic capacity . Due to an abnormal folding, the lytic activity of the mutant protein on the target cells was found to decrease 10-fold in comparison with that of the WT protein. However, the authors of the only studies available so far on this topic stated that the p.Ala91Val mutation alone did not suffice to cause the development of FHL type 2. Another event seems to be necessary to produce clinically significant effects. p.Ala91Val PRF1 may act as a synergistic factor with other genetic mutations predisposing patients to a larger range of cancer types such as renal cancer and CML, and these mutations may be involved in common immune surveillance and tumor escape mechanisms.
p.Ala91Val is the most common amino acid substitution identified in perforin, where the allele frequency ranges between 3% and 17%. It has long been a highly controversial case of polymorphism. It is an unusual case because it significantly affects the stability and the cytolytic activity of perforin due to the incorrect folding of the protein. It has been established that the homozygous mutation p.Ala91Val was carried by a large healthy population in whom perforin wild type function was preserved despite the presence of the p.Ala91Val substitution.
The variant p.Ala211Val was described in 5 members of one consanguineous Tunisian family; two of them were second cousins suffering from Hodgkin's Lymphoma. They died young. The probands were found to carry this germline mutation in the heterozygous state. The other family members are disease free mutation carriers. The fact that this new p.Ala211Val allele was not detected in any of the 200 control chromosomes screened suggests the pathogenicity of this mutation. However, the sequence alteration identified might also constitute a rare and hitherto undescribed polymorphism. Nonetheless, the present functional studies did not yield any evidence of altered PRF1 function in cells with overexpressed PRF1. This PRF1 variant does not predispose its carriers to hematological malignancies.
The results obtained in this study show that monoallelic PRF1 variants alone cannot be used as prognostic factors. However, these variants might contribute to the background of genetic factors which have yet to be identified. The new p.Ala211Val seems rather to be a rare allele.
In the cohort studied here, no PRF1 mutations predisposing their carriers to the development of hematological malignancies were identified. It is possible, however, that a heterozygous variant of PRF1 might act as an inherited risk factor in addition to other genetic variations (somatic or constitutional variations in a second allele) and/or in the presence of environmental factors. Epigenetic modifications have been described in mammalian genomes which have profound effects on gene expression. It has been established, for example, that the methylation of cytosine residues at CpG dinucleotides in the promoter region favors tumor development . The CpG island hypermethylation profile is specific to certain subtypes of leukemia and lymphoma . However, very little is known about the epigenetic of PRF1. Lu et al  have established that DNA methylation and chromatin structure participate in the regulation of PRF1 expression, whereas Gao et al  have described how demethylating treatment can suppress NK cell cytolytic activity. It would be interesting to investigate the epigenetic of PRF1 to complete our study.
As immune surveillance is believed to play a major role in preventing tumor growth and/or the development of autoimmune disorders, the range of clinical manifestations associated with perforin mutations may be wider than suspected so far. Additional still undiscovered genetic defects, or possibly even environmental factors may possibly contribute along with the PRF1 variant to predispose carriers of this mutation to the development of familial hematological malignancies. We now intend to screen PRF1 in tumoral DNAs and extend our investigations to other cooperating genes. Lymphocyte mediated apoptosis involves many molecules such as Fas, Fas Ligand, Caspase 8 and Caspase10. Together with PRF1, these genes are excellent candidates, which we are investigating and currently analyzing in familial cancer aggregations including hematological malignancies.
The identification of predisposing genetic factors would help to develop methods of genetic counseling and genetic testing for patients with familial hematological malignancies. Inherited molecular markers make it possible to identify these high-risk individuals and the family members at risk, as well as to enroll them in appropriate screening protocols.
Acknowledgements and Funding
This work was supported by la Société Française d'Hématologie, le groupe Génétique et Cancer and l'Institut National du Cancer INCa and by the Ministère de l'Enseignement Supérieur et de la Recherche Scientifique in Tunisia. It was part of the GenHem INSERM/DGRS project (2010-2011). We would like to thank Valérie Delague for helpful discussions, Institut Paoli Calmettes and Pr Patrice Viens.
- Grakoui A, Bromley SK, Sumen C, Davis MM, Shaw AS, Allen PM, Dustin ML: The immunological synapse: a molecular machine controlling T cell activation. Science 1999, 285: 221–227. 10.1126/science.285.5425.221View ArticlePubMedGoogle Scholar
- Stinchcombe JC, Bossi G, Booth S, Griffiths GM: The immunological synapse of CTL contains a secretory domain and membrane bridges. Immunity 2001, 15: 751–761. 10.1016/S1074-7613(01)00234-5View ArticlePubMedGoogle Scholar
- Lieberman J: The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal. Nat Rev Immunol 2003, 3: 361–370. 10.1038/nri1083View ArticlePubMedGoogle Scholar
- Russell JH, Ley TJ: Lymphocyte-mediated cytotoxicity. Annu Rev Immunol 2002, 20: 323–370. 10.1146/annurev.immunol.20.100201.131730View ArticlePubMedGoogle Scholar
- Bolitho P, Voskoboinik I, Trapani JA, Smyth MJ: Apoptosis induced by the lymphocyte effector molecule perforin. Curr Opin Immunol 2007, 19: 339–347. 10.1016/j.coi.2007.04.007View ArticlePubMedGoogle Scholar
- Stepp SE, Dufourcq-Lagelouse R, Le Deist F, Bhawan S, Certain S, Mathew PA, Henter JI, Bennett M, Fischer A, de Saint Basile G, Kumar V: Perforin gene defects in familial hemophagocytic lymphohistiocytosis. Science 1999, 286: 1957–1959. 10.1126/science.286.5446.1957View ArticlePubMedGoogle Scholar
- Goransdotter Ericson K, Fadeel B, Nilsson-Ardnor S, Soderhall C, Samuelsson A, Janka G, Schneider M, Gurgey A, Yalman N, Revesz T, et al.: Spectrum of perforin gene mutations in familial hemophagocytic lymphohistiocytosis. Am J Hum Genet 2001, 68: 590–597. 10.1086/318796View ArticlePubMedPubMed CentralGoogle Scholar
- Ueda I, Morimoto A, Inaba T, Yagi T, Hibi S, Sugimoto T, Sako M, Yanai F, Fukushima T, Nakayama M, et al.: Characteristic perforin gene mutations of haemophagocytic lymphohistiocytosis patients in Japan. Br J Haematol 2003, 121: 503–510. 10.1046/j.1365-2141.2003.04298.xView ArticlePubMedGoogle Scholar
- Suga N, Takada H, Nomura A, Ohga S, Ishii E, Ihara K, Ohshima K, Hara T: Perforin defects of primary haemophagocytic lymphohistiocytosis in Japan. Br J Haematol 2002, 116: 346–349. 10.1046/j.1365-2141.2002.03266.xView ArticlePubMedGoogle Scholar
- Molleran Lee S, Villanueva J, Sumegi J, Zhang K, Kogawa K, Davis J, Filipovich AH: Characterisation of diverse PRF1 mutations leading to decreased natural killer cell activity in North American families with haemophagocytic lymphohistiocytosis. J Med Genet 2004, 41: 137–144. 10.1136/jmg.2003.011528View ArticlePubMedGoogle Scholar
- Kogawa K, Lee SM, Villanueva J, Marmer D, Sumegi J, Filipovich AH: Perforin expression in cytotoxic lymphocytes from patients with hemophagocytic lymphohistiocytosis and their family members. Blood 2002, 99: 61–66. 10.1182/blood.V99.1.61View ArticlePubMedGoogle Scholar
- Ishii E, Ohga S, Imashuku S, Kimura N, Ueda I, Morimoto A, Yamamoto K, Yasukawa M: Review of hemophagocytic lymphohistiocytosis (HLH) in children with focus on Japanese experiences. Crit Rev Oncol Hematol 2005, 53: 209–223. 10.1016/j.critrevonc.2004.11.002View ArticlePubMedGoogle Scholar
- Imashuku S, Ueda I, Teramura T, Mori K, Morimoto A, Sako M, Ishii E: Occurrence of haemophagocytic lymphohistiocytosis at less than 1 year of age: analysis of 96 patients. Eur J Pediatr 2005, 164: 315–319. 10.1007/s00431-005-1636-9View ArticlePubMedGoogle Scholar
- Feldmann J, Le Deist F, Ouachee-Chardin M, Certain S, Alexander S, Quartier P, Haddad E, Wulffraat N, Casanova JL, Blanche S, et al.: Functional consequences of perforin gene mutations in 22 patients with familial haemophagocytic lymphohistiocytosis. Br J Haematol 2002, 117: 965–972. 10.1046/j.1365-2141.2002.03534.xView ArticlePubMedGoogle Scholar
- Clementi R, zur Stadt U, Savoldi G, Varoitto S, Conter V, De Fusco C, Notarangelo LD, Schneider M, Klersy C, Janka G, et al.: Six novel mutations in the PRF1 gene in children with haemophagocytic lymphohistiocytosis. J Med Genet 2001, 38: 643–646. 10.1136/jmg.38.9.643View ArticlePubMedPubMed CentralGoogle Scholar
- Katano H, Ali MA, Patera AC, Catalfamo M, Jaffe ES, Kimura H, Dale JK, Straus SE, Cohen JI: Chronic active Epstein-Barr virus infection associated with mutations in perforin that impair its maturation. Blood 2004, 103: 1244–1252.View ArticlePubMedGoogle Scholar
- Clementi R, Emmi L, Maccario R, Liotta F, Moretta L, Danesino C, Arico M: Adult onset and atypical presentation of hemophagocytic lymphohistiocytosis in siblings carrying PRF1 mutations. Blood 2002, 100: 2266–2267. 10.1182/blood-2002-04-1030View ArticlePubMedGoogle Scholar
- Clementi R, Dagna L, Dianzani U, Dupre L, Dianzani I, Ponzoni M, Cometa A, Chiocchetti A, Sabbadini MG, Rugarli C, et al.: Inherited perforin and Fas mutations in a patient with autoimmune lymphoproliferative syndrome and lymphoma. N Engl J Med 2004, 351: 1419–1424. 10.1056/NEJMoa041432View ArticlePubMedGoogle Scholar
- Clementi R, Locatelli F, Dupre L, Garaventa A, Emmi L, Bregni M, Cefalo G, Moretta A, Danesino C, Comis M, et al.: A proportion of patients with lymphoma may harbor mutations of the perforin gene. Blood 2005, 105: 4424–4428. 10.1182/blood-2004-04-1477View ArticlePubMedGoogle Scholar
- Bolitho P, Street SE, Westwood JA, Edelmann W, Macgregor D, Waring P, Murray WK, Godfrey DI, Trapani JA, Johnstone RW, Smyth MJ: Perforin-mediated suppression of B-cell lymphoma. Proc Natl Acad Sci USA 2009, 106: 2723–2728. 10.1073/pnas.0809008106View ArticlePubMedPubMed CentralGoogle Scholar
- Corthay A, Skovseth DK, Lundin KU, Rosjo E, Omholt H, Hofgaard PO, Haraldsen G, Bogen B: Primary antitumor immune response mediated by CD4+ T cells. Immunity 2005, 22: 371–383. 10.1016/j.immuni.2005.02.003View ArticlePubMedGoogle Scholar
- Street SE, Hayakawa Y, Zhan Y, Lew AM, MacGregor D, Jamieson AM, Diefenbach A, Yagita H, Godfrey DI, Smyth MJ: Innate immune surveillance of spontaneous B cell lymphomas by natural killer cells and gammadelta T cells. J Exp Med 2004, 199: 879–884. 10.1084/jem.20031981View ArticlePubMedPubMed CentralGoogle Scholar
- Zitvogel L, Tesniere A, Kroemer G: Cancer despite immunosurveillance: immunoselection and immunosubversion. Nat Rev Immunol 2006, 6: 715–727. 10.1038/nri1936View ArticlePubMedGoogle Scholar
- Kagi D, Vignaux F, Ledermann B, Burki K, Depraetere V, Nagata S, Hengartner H, Golstein P: Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity. Science 1994, 265: 528–530. 10.1126/science.7518614View ArticlePubMedGoogle Scholar
- Smyth MJ, Thia KY, Cretney E, Kelly JM, Snook MB, Forbes CA, Scalzo AA: Perforin is a major contributor to NK cell control of tumor metastasis. J Immunol 1999, 162: 6658–6662.PubMedGoogle Scholar
- Smyth MJ, Thia KY, Street SE, MacGregor D, Godfrey DI, Trapani JA: Perforin-mediated cytotoxicity is critical for surveillance of spontaneous lymphoma. J Exp Med 2000, 192: 755–760. 10.1084/jem.192.5.755View ArticlePubMedPubMed CentralGoogle Scholar
- van den Broek ME, Kagi D, Ossendorp F, Toes R, Vamvakas S, Lutz WK, Melief CJ, Zinkernagel RM, Hengartner H: Decreased tumor surveillance in perforin-deficient mice. J Exp Med 1996, 184: 1781–1790. 10.1084/jem.184.5.1781View ArticlePubMedGoogle Scholar
- Yuille MR, Matutes E, Marossy A, Hilditch B, Catovsky D, Houlston RS: Familial chronic lymphocytic leukaemia: a survey and review of published studies. Br J Haematol 2000, 109: 794–799. 10.1046/j.1365-2141.2000.02111.xView ArticlePubMedGoogle Scholar
- Houlston RS, Catovsky D, Yuille MR: Genetic susceptibility to chronic lymphocytic leukemia. Leukemia 2002, 16: 1008–1014. 10.1038/sj.leu.2402538View ArticlePubMedGoogle Scholar
- Bielawski K, Zaczek A, Lisowska U, Dybikowska A, Kowalska A, Falkiewicz B: The suitability of DNA extracted from formalin-fixed, paraffin-embedded tissues for double differential polymerase chain reaction analysis. Int J Mol Med 2001, 8: 573–578.PubMedGoogle Scholar
- Greer CE, Wheeler CM, Manos MM: Sample preparation and PCR amplification from paraffin-embedded tissues. PCR Methods Appl 1994, 3: S113–122.View ArticlePubMedGoogle Scholar
- Tavtigian SV, Deffenbaugh AM, Yin L, Judkins T, Scholl T, Samollow PB, de Silva D, Zharkikh A, Thomas A: Comprehensive statistical study of 452 BRCA1 missense substitutions with classification of eight recurrent substitutions as neutral. J Med Genet 2006, 43: 295–305.View ArticlePubMedGoogle Scholar
- Tong W, Li L, Weng Z: Computational prediction of binding hotspots. Conf Proc IEEE Eng Med Biol Soc 2004, 4: 2980–2983.PubMedGoogle Scholar
- Cartegni L, Wang J, Zhu Z, Zhang MQ, Krainer AR: ESEfinder: A web resource to identify exonic splicing enhancers. Nucleic Acids Res 2003, 31: 3568–3571. 10.1093/nar/gkg616View ArticlePubMedPubMed CentralGoogle Scholar
- Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR: An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet 2006, 15: 2490–2508. 10.1093/hmg/ddl171View ArticlePubMedGoogle Scholar
- Fairbrother WG, Yeh RF, Sharp PA, Burge CB: Predictive identification of exonic splicing enhancers in human genes. Science 2002, 297: 1007–1013. 10.1126/science.1073774View ArticlePubMedGoogle Scholar
- Voskoboinik I, Thia MC, De Bono A, Browne K, Cretney E, Jackson JT, Darcy PK, Jane SM, Smyth MJ, Trapani JA: The functional basis for hemophagocytic lymphohistiocytosis in a patient with co-inherited missense mutations in the perforin (PFN1) gene. J Exp Med 2004, 200: 811–816. 10.1084/jem.20040776View ArticlePubMedPubMed CentralGoogle Scholar
- Voskoboinik I, Thia MC, Trapani JA: A functional analysis of the putative polymorphisms A91V and N252S and 22 missense perforin mutations associated with familial hemophagocytic lymphohistiocytosis. Blood 2005, 105: 4700–4706. 10.1182/blood-2004-12-4935View ArticlePubMedGoogle Scholar
- Clementi R, Chiocchetti A, Cappellano G, Cerutti E, Ferretti M, Orilieri E, Dianzani I, Ferrarini M, Bregni M, Danesino C, et al.: Variations of the perforin gene in patients with autoimmunity/lymphoproliferation and defective Fas function. Blood 2006, 108: 3079–3084. 10.1182/blood-2006-02-001412View ArticlePubMedGoogle Scholar
- Solomou EE, Gibellini F, Stewart B, Malide D, Berg M, Visconte V, Green S, Childs R, Chanock SJ, Young NS: Perforin gene mutations in patients with acquired aplastic anemia. Blood 2007, 109: 5234–5237. 10.1182/blood-2006-12-063495View ArticlePubMedPubMed CentralGoogle Scholar
- Voskoboinik I, Sutton VR, Ciccone A, House CM, Chia J, Darcy PK, Yagita H, Trapani JA: Perforin activity and immune homeostasis: the common A91V polymorphism in perforin results in both presynaptic and postsynaptic defects in function. Blood 2007, 110: 1184–1190. 10.1182/blood-2007-02-072850View ArticlePubMedGoogle Scholar
- Jones PA, Baylin SB: The fundamental role of epigenetic events in cancer. Nat Rev Genet 2002, 3: 415–428.View ArticlePubMedGoogle Scholar
- Esteller M, Corn PG, Baylin SB, Herman JG: A gene hypermethylation profile of human cancer. Cancer Res 2001, 61: 3225–3229.PubMedGoogle Scholar
- Lu Q, Wu A, Ray D, Deng C, Attwood J, Hanash S, Pipkin M, Lichtenheld M, Richardson B: DNA methylation and chromatin structure regulate T cell perforin gene expression. J Immunol 2003, 170: 5124–5132.View ArticlePubMedGoogle Scholar
- Gao XN, Lin J, Wang LL, Yu L: Demethylating treatment suppresses natural killer cell cytolytic activity. Mol Immunol 2009, 46: 2064–2070. 10.1016/j.molimm.2009.02.033View ArticlePubMedGoogle Scholar
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