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Screening for ovarian cancer in women with varying levels of risk, using annual tests, results in high recall for repeat screening tests
- Marielle AE Nobbenhuis1Email author,
- Elizabeth Bancroft2,
- Eleanor Moskovic3,
- Fiona Lennard2,
- Paul Pharoah4,
- Ian Jacobs5,
- Ann Ward2,
- Desmond PJ Barton1,
- Thomas EJ Ind1,
- John H Shepherd1,
- Jane E Bridges1,
- Martin Gore1,
- Chris Haracopos2,
- Susan Shanley2,
- Audrey Ardern-Jones2,
- Sarah Thomas2 and
- Ros Eeles2
© Nobbenhuis et al; licensee BioMed Central Ltd. 2011
Received: 30 August 2011
Accepted: 23 November 2011
Published: 23 November 2011
We assessed ovarian cancer screening outcomes in women with a positive family history of ovarian cancer divided into a low-, moderate- or high-risk group for development of ovarian cancer.
545 women with a positive family history of ovarian cancer referred to the Ovarian Screening Service at the Royal Marsden Hospital, London from January 2000- December 2008 were included. They were stratified into three risk-groups according to family history (high-, moderate- and low-risk) of developing ovarian cancer and offered annual serum CA 125 and transvaginal ultrasound screening. The high-risk group was offered genetic testing.
The median age at entry was 44 years. The number of women in the high, moderate and low-risk groups was 397, 112, and 36, respectively. During 2266 women years of follow-up two ovarian cancer cases were found: one advanced stage at her fourth annual screening, and one early stage at prophylactic bilateral salpingo-oophorectomy (BSO). Prophylactic BSO was performed in 138 women (25.3%). Forty-three women had an abnormal CA125, resulting in 59 repeat tests. The re-call rate in the high, moderate and low-risk group was 14%, 3% and 6%. Equivocal transvaginal ultrasound results required 108 recalls in 71 women. The re-call rate in the high, moderate, and low-risk group was 25%, 6% and 17%.
No early stage ovarian cancer was picked up at annual screening and a significant number of re-calls for repeat screening tests was identified.
Keywordsovarian cancer screening transvaginal ultrasound serum CA125 BRCA gene mutation bilateral salpingo-oophorectomy
Primary ovarian cancer is the fourth most common cause of cancer-related death in the United Kingdom with 4500 deaths each year . The identification of BRCA1 and BRCA2 gene mutations and their relationship to ovarian cancer has resulted in an awareness of family history in many women with ovarian cancer [2–5]. Several studies have investigated the role of screening in women with a positive family history of ovarian cancer. Unfortunately, no clear evidence of the efficacy of different screening strategies has been documented [6–8]. The normal life-time risk of developing epithelial ovarian cancer in the UK is estimated to be 1-1.5%. This risk is higher in women with a family history of ovarian cancer and increases with the number of affected relatives and the closeness of the relationship . In women with a known BRCA1 or BRCA2 gene mutation the cumulative life-time risk of developing ovarian cancer can be as high as 60% and 27%, respectively, although it may be between 11-39% if the family history is less strong [4, 10, 11]. The prognosis of ovarian cancer is good when diagnosed at an early stage (FIGO stage I-II) with 80--95% 5-year survival. However, the majority of ovarian cancer cases (70%) are detected at an advanced stage with poor survival .
For women at high-risk of developing ovarian cancer, management options still include a) ovarian cancer screening by CA125 measurement and or transvaginal ultrasound, with the intention to reduce mortality by detecting ovarian cancer at an early stage, or b) prophylactic bilateral salpingo-oophorectomy (BSO). The optimal management policy in women with moderate or low-risk of developing ovarian cancer remains unclear.
In this study, we assessed ovarian cancer screening outcomes in women with a positive family history of ovarian cancer stratified in a low, moderate or high-risk group of developing ovarian cancer. We defined the number of ovarian cancer cases identified, the number of re-calls because of abnormal test results, and present the characteristics of women who underwent prophylactic BSO.
Increased risk criteria in women with a family history of breast and ovarian cancer 
Family contains 2 or more individuals with ovarian cancer who are first degree relatives
Family contains 1 individual with ovarian cancer and 1 individual with breast cancer diagnosed < 50 years who are first degree relatives
Family contains 1 individual with ovarian cancer and 2 individuals with breast cancer diagnosed < 60 years who are connected by first degree relationships
Family contains an affected individual with a mutation in one of the known ovarian cancer predisposition genes BRCA1, BRCA2, MSH2, MSH6, MLH1, PMS1 or PMS2
Family contains 3 individuals with colorectal cancer with at least 1 case diagnosed before 50 years and 1 case of ovarian cancer. All individuals are connected by first degree relationships
Criteria 1,2 and 3 can be modified where paternal transmission is occurring, i.e. families where affected relatives are related by second degree through an unaffected intervening male relative and a sister is affected are eligible
The provision of ovarian screening was discontinued at The Royal Marsden NHS Foundation Trust in 2004 as screening was concentrated in other centres, if appropriate; pending the results of studies of screening in high-risk families (the UKFOCSS study). All patients had a full family history taken out to third degree relatives and their risk was reviewed by a cancer geneticist (RE). Those patients who had been assessed as being at high-risk for ovarian cancer were offered participation in the UKFOCSS study (UK Familial Ovarian Cancer Screening Study). Those who were been assessed as being at moderate-risk were referred to St Georges Hospital, London, one of the very few NHS Gynaecology Departments which still offered ovarian cancer screening outside a research study to moderate-risk women, or to a local hospital if this was more convenient for the woman. All other patients were considered low-risk, indicating that some women formerly considered at increased risk were reclassified as low-risk and advised that there was no indication for further screening and were discharged. From 2004, new patients were still initially assessed in our clinic but referred according to risk group. Inclusion in the study ended in December 2008. The total number of prophylactic BSO was recorded as were the number of repeat CA125 measurements and transvaginal ultrasound visits due to abnormal findings. The expected number of ovarian cancers to be detected in this study was calculated, divided into women years tested by risk category and age group (using incidence rates of Antoniou et al, 2003)  Statistical analysis was performed using SPSS software (version 17.0, SPSS Inc. Chicago).
As this was a clinical audit, a formal ethical review was not required. According to hospital protocol the study was reviewed and approved by the Royal Marsden Clinical Audit Committee.
Patient characteristics at baseline with results of genetic testing in the high-risk group
< 35 years
≥ 50 years
At baseline, the majority of women was in the high-risk group n = 397 (73%). The moderate- and low-risk group consisted of 112 (21%) and 36 (6.6%) women, respectively. In the high-risk group 140 women (35.3%) underwent genetic testing and in 106 (76%) a genetic mutation was identified. Among these women, 58 (55%) women were BRCA1 gene mutation carriers and 45 (43%) women had a BRCA2 gene mutation. In 2 (1.9%) an MLH1 gene defect mutation was identified. One (0.9%) woman was diagnosed with both a BRCA1 and BRCA 2 gene mutation. This 69 year old lady underwent a hysteroscopy, dilatation and curettage for an endometrial polyp which revealed a grade 3 endometrioid adenocarcinoma of the endometrium. She underwent a laparoscopic hysterectomy and bilateral salpingo-oophorectomy showing FIGO stage 1B endometrial cancer. Her ovaries and fallopian tubes were histologically normal.
In 2 women, epithelial ovarian cancer was diagnosed. The first case, a 50-year-old BRCA1 gene mutation carrier, presented at her fourth annual screening visit with an elevated CA125 of 3874 kU/L and bilateral complex ovarian masses with ascites on transvaginal ultrasound. She was asymptomatic. Her previous screening CA125 levels and transvaginal ultrasounds did not show any abnormalities. At her third annual screening visit, 14 months before she was diagnosed with ovarian cancer, her CA125 was 8 kU/L She underwent optimal debulking surgery and the final histopathology revealed Stage 3C poorly differentiated serous papillary adenocarcinoma of the ovary. The second ovarian cancer case was found in a 40 year old BRCA1 gene mutation carrier. She underwent a routine prophylactic BSO. The adnexa were grossly normal but the histology revealed Stage 1A poorly differentiated serous adenocarcinoma of the ovary. Her preoperative CA125 was 30 kU/L and no abnormalities were seen on transvaginal ultrasound. She had been undergoing annual screening for 5 years. Both patients are still alive after 6 and 4 years of follow-up, respectively.
Characteristics of 138 women who underwent prophylactic bilateral salpingo-oophorectomy
Ovarian stromal hyperplasia
A total of 2266 women years of follow-up were identified. The majority of women years (1623 women years) were in the high-risk group, with 522 and 121 women years in the moderate- and low-risk group, respectively. A total of 3.89 (95% C.I. 0.22-7.22) ovarian cancer cases were expected in the study population with 3.11, 0.68 and 0.11 cases in the high-, moderate-, and low-risk groups, respectively.
Number of patients with abnormal ovarian screening test results and number of repeat tests among different risk groups
Risk group (Total no. pts)
No of pts with abnormal test result
No of repeat tests
Prophylactic BSO because of abnormal test
High risk (397)
Moderate risk (112)
Low risk (36)
Forty-three women had an abnormal CA125 measurement, resulting in a total of 59 repeat tests. The majority of elevated CA125 was seen in the high-risk group. Only one moderate-risk woman underwent a prophylactic BSO because of 2 consecutive elevated CA125 measurements (CA125 was 64 and 57 kU/l, respectively). Her transvaginal ultrasounds were normal. No abnormalities were found on final histopathology. In all, but three women, CA125 results came back to normal. Among these three women, one was diagnosed with advanced ovarian cancer, one woman was pregnant and the third diagnosed with recurrent breast cancer.
In this retrospective study we assessed ovarian cancer screening outcomes in 545 women with a positive history of ovarian cancer stratified into a low, moderate, or high-risk group of developing ovarian cancer.
A total of 2 (0.4%) ovarian cancer cases were identified. None of them were found during screening. Most studies looking at the effectiveness of ovarian cancer screening found a higher incidence of ovarian cases of about 1-3% [7, 14]. Although our number of identified ovarian cancer cases was low in comparison with these studies, the annual screening detected the number of cases expected for a population of this age, risk spectrum and duration of follow-up. One reason for the low incidence of ovarian cancer is that our cohort included moderate- and low-risk women. Laframboise et al. reported 1 ovarian cancer in a series of 311 women of which only 10% were BRCA gene mutation carriers .
In our high-risk group 35% underwent genetic testing and in 76% a genetic mutation was identified. The reason for this high percentage could be the high proportion of Ashkenazi women (30%) and women with a previous history of breast cancer (47%) in this group.
Prophylactic BSO was performed in 25% of women and in one case early stage ovarian cancer was found, but on histology only. She had normal pre-operative screening results. Transvaginal ultrasound and CA125 measurements as screening tests are known to have their limitations. High false-positive rates have been identified, especially in pre-menopausal women, resulting in a high number of re-call visits, anxiety through equivocal test results, and even, unnecessary surgical intervention [14–16]. In our study we found at least one elevated CA125 in 43 women (8%) and at least one abnormal transvaginal ultrasound in 71 women (13%). In the majority of cases the repeat tests showed normal results, although in 8 women a BSO was performed due to a persistently abnormal test result. No ovarian cancer was found in these women. Other studies have shown a significant number of abnormal CA125 results and transvaginal ultrasounds during surveillance . Oei et al. screened 512 high-risk women and found abnormalities in 21% of all transvaginal ultrasounds and in 4% of all CA125 measurements . The majority of these abnormal results disappeared within 3-6 months, resulting in a high number of unnecessary re-calls. Meeuwissen et al. observed abnormal results in 19% of 383 high-risk women, with 64% of them returning to normal spontaneously .
The limitation of our study is that it has not been conducted in the form of a robust prospective protocol, but was an analysis of the clinical management guidelines at the time. Screening results were interpreted and acted on by clinicians involved with no defined protocol other than using the RMI only. Our study revealed a significant number of re-calls for repeat screening tests. No early stage ovarian cancer was identified at annual screening by serum CA125 and/or transvaginal ultrasound. Of the two ovarian cases found, one early stage ovarian cancer was found at prophylactic BSO. The other case was found during screening and presented with an advanced ovarian cancer. Both patients had been under surveillance for more than 4 years and had no abnormal results in their previous annual screening rounds. Several studies have already reported that annual screening of high-risk women will not reduce mortality from ovarian cancer [6, 19]. A large study of 3532 high-risk women screened annually with transvaginal ultrasound and CA125 did not show any benefit in survival; the majority of ovarian cancer cases was found in an advanced stage . Another study including 241 BRCA positive women who underwent ovarian surveillance concluded low sensitivity and positive predictive values of ovarian screening modalities and no additional benefit from screening .
More sophisticated screening criteria have been developed since the start of our study and we are awaiting the results of a large UK multi-centre trial, the UKFOCCS trial to clarify the role of different algorithms of ovarian screening in women with an increased genetic risk to assess if this improves upon the annual re-call algorithm. Since no effective screening programme has yet been identified, prophylactic BSO should be advised to all high-risk women after they completed their family which will reduce the risk of ovarian cancer associated with BRCA 1 or BRCA 2 mutation by 70-96% [20–22].
In conclusion, this study assessed the role of annual ovarian screening in women with a family history of ovarian cancer stratified into 3 different risk-groups. No early stage ovarian cancer was picked up at annual screening. This approach to screening led to a significant number of re-calls for repeat screening tests.
We would like to thank the women who took part in the ovarian cancer screening programme at The Royal Marsden. We acknowledge support of the NIHR to the Biomedical Research Centre at The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust and Professor Tom Bourne for taking over a part of our patients for screening at St Georges Hospital, London.
- Office for National Statistics: Cancer Statistics registrations: Registrations of cancer diagnosed in 2005. England; 2008. Series MB1 no.36.Google Scholar
- Miki Y, Swensen J, Shattuck-Eidens D, Futreal P, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett M, Ding W, Bell R, Rosenthal J, Hussey C, Tran T, McClure M, Frye C, Hattier T, Phelps R, Haugen-Strano A, Katcher H, Yakumo K, Gholami Z, Shaffer D, Stone S, Bayer S, Wray C, Bogden R, Dayananth P, Ward J, Tonin P, et al.: A strong candidate for the breast and ovarian cancer susceptibility gene BRCA 1 . Science 1994, 266: 66–71. 10.1126/science.7545954View ArticlePubMedGoogle Scholar
- Wooster R, Bignell G, Lancaster J, Swift S, Seal S, Mangion J, Collins N, Gregory S, Gumbs C, Micklem G: Identification of the breast cancer susceptibility gene BRCA2. Nature 1995, 379: 789–92.View ArticleGoogle Scholar
- Antoniou A, Pharoah PDP, Narod S, Risch HA, Eyfjord JE, Hopper JL, Loman N, Olsson H, Johannsson O, Borg Å, Pasini B, Radice P, Manoukian S, Eccles DM, Tang N, Olah E, Anton-Culver H, Warner E, Lubinski J, Gronwald J, Gorski B, Tulinius H, Thorlacius S, Eerola H, Nevanlinna H, Syrjäkoski K, Kallioniemi OP, Thompson D, Evans C, Peto J, Lalloo F, Evans DG, Easton DF: Average risks of breast and ovarian cancer associated with BRCA1 or BRCA2 mutations detected in case series unselected for family history; A combined analysis of 22 studies. Am J Hum Genet 2003, 72: 1117–30. 10.1086/375033View ArticlePubMedPubMed CentralGoogle Scholar
- King MC, Marks JH, Mandell JB: Breast and ovarian cancer risks due to inherited mutations in BRCA1 and BRCA2 . Science 2003, 24: 643–6.View ArticleGoogle Scholar
- Hermsen BBJ, Olivier RI, Verheijen RHM, van Beurden M, de Hullu JA, Massuger LF, Burger CW, Brekelmans CT, Mourits MJ, de Bock GH, Gaarenstroom KN, van Boven HH, Mooij TM, Rookus MA: No efficacy of annual gynaecological screening in BRCA1/2 mutation carriers; an observational follow-up study. Br J Cancer 2007, 96: 1335–42.PubMedPubMed CentralGoogle Scholar
- Evans DG, Gaarenstroom KN, Stirling D, Shenton A, Maehle L, Dorum A, Steel M, Lalloo F, Apold J, Porteous ME, Vasen HFA, van Asperen CJ, Moller P: Screening for familial ovarian cancer: poor survival of BRCA1/2 related cancers. J Med Genet 2009, 46: 593–7. 10.1136/jmg.2008.058248View ArticlePubMedGoogle Scholar
- Van der Velde NM, Mourits MJE, Aarts HJG, de Vries J, Leegte BK, Dijkhuis G, Oosterwijk JC, de Bock GH: Time to stop ovarian cancer screening in BRCA1/2 mutation carriers? Int J Cancer 2009, 124: 919–23. 10.1002/ijc.24038View ArticlePubMedGoogle Scholar
- Stratton JF, Pharoah P, Smith SK, Easton D, Ponder BA: A systematic review and meta-analysis of family history and risk of ovarian cancer. Br J Obstet Gynaecol 1998, 105: 493–9. 10.1111/j.1471-0528.1998.tb10148.xView ArticlePubMedGoogle Scholar
- Chen S, Iversen ES, Friebel T, Finkelstein D, Weber B, Eisen A, Peterson L, Schildkraut J, Isaacs C, Peshkin B, Corio C, Leondaridis L, Tomlinson G, Dutson D, Kerber R, Amos C, Strong L, Berry D, Euhus D, Parmigiani G: Characterization of BRCA1 and BRCA2 mutations in a large United States sample. J Clin Oncol 2006, 24: 863–71. 10.1200/JCO.2005.03.6772View ArticlePubMedPubMed CentralGoogle Scholar
- Ford D, Easton DF, Stratton M, Narod S, Goldgar D, Devilee P, Bishop DT, Weber B, Lenoir G, Chang-Claude J, Sobol H, Teare MD, Struewing J, Arason A, Scherneck S, Peto J, Rebbeck TR, Tonin P, Neuhausen S, Barkardottir R, Eyfjord J, Lynch H, Ponder BAJ, Gayther SA, Birch JM, Lindblom A, Stoppa-Lyonnet D, Bignon Y, Borg A, Hamann U, et al.: Genetic heterogeneity and penetrance analysis of the BRCA1 and BRCA2 genes in breast cancer families. Am J Hum Genet 1998, 62: 676–89. 10.1086/301749View ArticlePubMedPubMed CentralGoogle Scholar
- Hennessy BT, Coleman RL, Markham M: Ovarian cancer. Lancet 2009, 374: 1371–82. 10.1016/S0140-6736(09)61338-6View ArticlePubMedGoogle Scholar
- McIntosh A, Shaw C, Evans G, Turnbull N, Bahar N, Barclay M, Easton D, Emery J, Gray J, Halpin J, Hopwood P, McKay J, Sheppard C, Sibbering M, Watson W, Walloo A, Hutchinson A: Clinical Guidelines Evidence Review for The Classification Care of Women at Risk of Familial Breast Cancer. In NICE Guideline CG014. London: National Collaborating Centre for Primary Care, University of Sheffield; 2004.Google Scholar
- Stirling D, Evans GR, Pichert G, Shenton A, Kirk EN, Rimmer S, Steel CM, Lawson S, Busby-Earle RMC, Walker J, Lalloo FI, Eccles DM, Lucassen AM, Porteous ME: Screening for familial ovarian cancer: Failure of current protocols to detect ovarian cancer at an early stage according to the International Federation of Gynecology and Obstetrics system. J Clin Oncol 2005, 23: 5588–96. 10.1200/JCO.2005.05.097View ArticlePubMedGoogle Scholar
- Laframboise S, Nedelcu R, Murphy J, Cole DEC, Rosen B: Use of CA-125 and ultrasound in high-risk women. Int J Gynecol Cancer 2002, 12: 86–91. 10.1046/j.1525-1438.2002.01055.xView ArticlePubMedGoogle Scholar
- Scheuer L, Kauff N, Robson M, Kelly B, Barakat R, Satagopan J, Ellis N, Hensley M, Boyd J, Borgen P, Norton L, Offit K: Outcome and preventive surgery and screening for breast and ovarian cancer in BRCA mutation carriers. J Clin Oncol 2002, 20: 1260–8. 10.1200/JCO.20.5.1260View ArticlePubMedGoogle Scholar
- Oei AL, Massuger LF, Bulten J, Ligtenberg MJ, Hoogerbrugge N, de Hullu JA: Surveillance of women at high risk for hereditary ovarian cancer is inefficient. Br J Cancer 2006, 94: 814–9. 10.1038/sj.bjc.6603015View ArticlePubMedPubMed CentralGoogle Scholar
- Meeuwissen PAM, Seynaeve C, Brekelmans CTM, Meijers-Heijboer HJ, Klijn JGM, Burger CW: Outcome of surveillance and prophylactic salpingo-oophorectomy in asymptomatic women at high risk for ovarian cancer. Gynecol Oncol 2005, 97: 476–82. 10.1016/j.ygyno.2005.01.024View ArticlePubMedGoogle Scholar
- Olivier RI, Lubsen-Brandsma MAC, Verhoef S, van Beurden M: CA125 and transvaginal ultrasound monitoring in high-risk women cannot prevent the diagnosis of advanced ovarian cancer. Gynecol Oncol 2006, 100: 20–6. 10.1016/j.ygyno.2005.08.038View ArticlePubMedGoogle Scholar
- Rebbeck TR, Lynch HT, Neuhausen SL, Narod SA, van 't Veer L, Garber JE, Evans G, Isaacs C, Daly MB, Matloff E, Olopade OI, Weber BL: Prophylactic oophorectomy in carriers of BRCA1 or BRCA2 mutations. N Engl J Med 2002, 346: 1616–22. 10.1056/NEJMoa012158View ArticlePubMedGoogle Scholar
- Finch A, Beiner M, Lubinski J, Lynch HT, Moller P, Rosen B, Murphy J, Ghadirian P, Friedman E, Foulkes WD, Kim-Sing C, Wagner T, Tung N, Couch F, Stoppa-Lyonnet D, Ainsworth P, Daly M, Pasini B, Gershoni-Baruch R, Eng C, Olopade O, McLennan J, Karlan B, Weitzel J, Sun P, Narod S: Salpingo-oophorectomy and the risk of ovarian, fallopian tube, and peritoneal cancers in women with a BRCA 1 or BRCA 2 mutation. JAMA 2006, 296: 185–92. 10.1001/jama.296.2.185View ArticlePubMedGoogle Scholar
- Domchek SM, Friebel TM, Singer CF, Evans DG, Lynch HT, Isaacs C, Garber JE, Neuhausen SL, Matloff E, Eeles R, Pitchert G, Van 't veer L, Tung N, Weitzel JN, Couch FJ, Rubinstein WS, Ganz PA, Daly MB, Olopade O, Tomlinson G, Schildkraut J, Blum JL, Rebbeck TE: Association of risk-reducing surgery in BRCA 1 or BRCA2 Mutation carriers with cancer risk and mortality. JAMA 2010, 304: 967–75. 10.1001/jama.2010.1237View ArticlePubMedPubMed CentralGoogle Scholar
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