- Open Access
© The Author(s) 2008
- Published: 15 June 2008
- Endometrial Cancer
- Lynch Syndrome
- Amsterdam Criterion
- Surveillance Protocol
- MSH6 Gene
The MSH6 gene in collaboration with MSH2, MLH1, MSH3, PMS1 and PMS2 genes is involved in one of the systems repairing the errors that arise during DNA replication, called the "methyl directed mismatch repair" system [1–3]. hMLH1 and hMSH2 mutations give rise most frequently to the classical Lynch syndrome (HNPCC) [4–6]. hMSH6 mutations often occur in clinically less typical HNPCC families, that do not fulfil the Amsterdam criteria [7–11]. So far more than 200 families with germline hMSH6 mutation have been described http://www.med.ca/MMRvariants.
Characteristic features of families with hMSH6 mutation are:
higher risk for colorectal cancer (~70% for men and ~30% for females), endometrial cancer (~70%) and also for ovarian, upper urinary tract, stomach and breast cancer ,
higher incidence of extracolonic cancers, when compared with HNPCC families ,
frequent left-sided localization of colorectal cancer .
Because the frequency of hMSH6 mutations in other groups is not precisely determined, we recommend the following diagnostic procedure for hMSH6 mutation detection:
selection of families with colorectal, endometrial, ovarian, urinary tract and/or stomach, breast cancer aggregation,
in cases of hMSH6-negative tumours, DHPLC/sequencing of the coding regions of the hMSH6 gene.
Among the reported hMSH6 mutations about 30 are recurrent, i.e. they are detected in more than one family [17–19]. Probably in the near future diagnostic tests will be described to allow cheap, simple and fast detection of mutations showing a "founder effect" characteristic for particular ethnic groups.
As yet a suitable surveillance programme in hMSH6 families based on prospective family trials is not available. In our centre we offer the following surveillance protocol:
colorectal cancer - colonoscopy every 2 to 3 years beginning at least 15 years before the earliest age of onset in the family,
endometrial cancer - annual transvaginal ultrasound scan starting at least 15 years before the earliest age of onset in the family,
screening for other cancers depending on the tumour spectrum in the family.
Because of the described higher risk of developing multiple (synchronous and metachronous) cancers and also endometrial cancer in females after age of 50 years in hMSH6 mutation carriers [12, 17, 20], it is recommended to take into consideration surgical treatment similar to that for patients with CRC associated with Lynch syndrome, i.e. colectomy with ileorectal anastomosis, extended by prophylactic hysterectomy and oophorectomy for women over 50 years old.
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