Skip to main content

The Pathology of Hereditary Breast Cancer


Several studies have demonstrated that familial breast cancers associated with BRCA1 or BRCA2 germline mutations differ in their morphological and immunohistochemical characteristics. Cancers associated with BRCA1 are poorly differentiated infiltrating ductal carcinomas (IDCs) with higher mitotic counts and pleomorphism and less tubule formation than sporadic tumours. In addition, more cases with the morphological features of typical or atypical medullary carcinoma are seen in these patients. Breast carcinomas from BRCA2 mutation carriers tend to be of higher grade than sporadic age-matched controls. Regarding immunophenotypic features. BRCA1 tumours have been found to be more frequently oestrogen receptor- (ER) and progesterone receptor-(PR) negative, and p53-positive than age-matched controls, whereas these differences are not usually found in BRCA2-associated tumours. A higher frequency and unusual location of p53 mutations have been described in BRCA1/2 carcinomas. Furthermore, BRCA1- and BRCA2-associated breast carcinomas show a low frequency of HER-2 expression. Recent studies have shown that most BRCA1 carcinomas belong to the basal cell phenotype, a subtype of high grade, highly proliferating ER/HER2-negative breast carcinoma characterized by the expression of basal or myoepithelial markers, such as basal keratins, P-cadherin, EGFR, etc. This phenotype occurs with a higher incidence in BRCA1 tumours than in sporadic carcinomas and is rarely found in BRCA2 carcinomas. Hereditary carcinomas not attributable to BRCA1/2 mutations have phenotypic similarities with BRCA2 tumours, but tend to be of lesser grade and lower proliferation index. The pathological features of hereditary breast cancer can drive specific treatment and influence the process of mutation screening.


It is currently estimated that 5-10% of all breast cancers are hereditary and attributable to mutations in several highly penetrant susceptibility genes, of which only two have been identified: BRCA1 (OMIM 113705) [1] and BRCA2 (OMIM 600185) [2]. Earlier estimates suggested that BRCA1 and BRCA2 mutations were responsible for 75% of site-specific breast cancer families and the majority of breast and ovarian cancer families [3, 4]. Recent data shows however that these percentages may have been overestimated and that the proportion of families classified due to mutations in BRCA1 or BRCA2 is much lower and strongly depends on the population analyzed [5] and the specific characteristics of the selected families [6, 7]. In fact, the percentage of high risk families associated with mutations in these genes is very similar (around 25%) in all series, including the one we have found in Spain [8, 9].

Genetic testing for BRCA1 and BRCA2 is expensive and time-consuming due to the large size of both genes, the absence of hot spots for mutations throughout their entire coding regions, and the low percentage of mutated cases. It is therefore important to find clinical or pathological factors that could suggest or exclude the presence of BRCA1 or BRCA2 mutations in a given patient.

The histopathology of BRCA-associated cancer has been studied by different groups; however the larger series are those reported by the Breast Cancer Linkage Consortium (BCLC) [1012]. These studies have demonstrated that cancer arising in carriers of mutations in either the BRCA1 or BRCA2 genes differs morphologically from sporadic breast cancers from age-matched controls [1114]. In addition, numerous immunohistochemical studies have tried to better characterize the differences between hereditary and sporadic tumours [10, 1518]. More recently, some reports have also described the pathology of hereditary breast cancer not attributable to BRCA1 or BRCA2 germline mutations. The purpose of this review is to present the histopathological characteristic of different genotypes of hereditary breast cancer. Special attention will be given to those characteristics that possibly impact on genetic testing, prognosis and treatment.

Histopathology of BRCA1 and BRCA2 breast cancer

In order to better understand the specific characteristics of hereditary breast cancer, the histopathological and immunohistochemical variables usually evaluated in sporadic breast cancer are also presented, since invasive breast carcinoma is a heterogeneous group of malignant epithelial tumours with a wide range of morphological phenotypes and specific histopathological types.

Histological type

Invasive ductal carcinoma (IDC) not otherwise specified (NOS) is the most common histological type among sporadic breast cancer, comprising 70-80% of all cases. The most frequent special histological types are invasive lobular carcinoma (5-15%), tubular carcinoma (2%), invasive cribriform carcinoma (0.8-3.5%), medullary carcinoma (1-7%), mucin producing carcinoma (2%), neuroendocrine tumours (2-5%), invasive micropapillar carcinoma (2%). Differences between series are related with the specific population studied or, more probably, with stringency in the application of diagnostic criteria.

Medullary carcinoma is a particular type of carcinoma characterized by the presence of solid sheets of large and pleomorphic cells with indistinct cell borders that lead to a syncytial appearance [19]. They are high-grade tumours with numerous mitosis, and sparse necrosis (<25%). The border of the tumour is well defined with a pushing edge. The stroma is a dense lymphocytic infiltrate. Despite being a high-grade tumour, it has been associated with a relatively favourable prognosis [1921]. Atypical medullary carcinoma is diagnosed when more than 25% of tumour is not classical medullary or the lymphoid infiltration is moderate or the circumscription is not complete.

IDC NOS is the most common histological type in all forms of hereditary breast cancer and it seems to be significantly more frequent in BRCA1 and BRCA2 mutation carriers than in non-carriers [22]. In addition, BRCA1 mutation carriers have a higher incidence of medullary carcinoma (13%) than BRCA2 mutation carriers (3%) and non-carriers (2%) [12]. When only IDC cases are compared, after excluding medullary carcinomas, BRCA1 tumours have more frequently a prominent lymphocytic infiltrate and pushing margins [11], some of the features that define the medullary histotype.

Regarding BRCA2 carcinomas, Marcus et al have reported a higher incidence of BRCA2 tumours belonging to a "tubular lobular group", including invasive lobular, tubular and cribriform carcinomas [23, 24]. Armes et al found that BRCA2 mutation carriers showed an excess of pleomorphic lobular and intraductal carcinomas [13]. However, other series have not found any statistically significant difference in the histological type of BRCA2 with respect to controls [11, 12, 25].

Histological grade

Breast carcinomas are routinely graded based on an assessment of tubule formation, nuclear pleomorphism and mitotic counts. This method of tumour graduation consists of scoring 1-3 for each factor. The grade is obtained by adding the three previous parameters and classifying the tumour as grade 1 (well differentiated or low-grade), grade 2 (moderately differentiated or intermediate-grade) and grade 3 (poorly differentiated or high-grade). This grading system has prognostic implications and the utility has been convincingly proved [26, 27].

BRCA1 tumours are more frequently high-grade (grade 3) tumours because they show less tubule formation, higher pleomorphism and a higher number of mitosis than sporadic age-matched breast cancer controls. Thus, the reported incidence of grade 3 tumours in BRCA1 mutation carriers ranged from 66% to 84% in different studies [12, 28, 29] while the proportion of grade 3 tumours in sporadic age-matched breast cancer controls was between 30% and 40% [12, 28, 29].

BRCA2 tumours tend to be of higher grade than sporadic controls, although this association is less strong than for BRCA1 cases. Most BRCA2 tumours are grade 2/3, and show less tubule formation but similar cellular pleomorphism and mitotic count than sporadic cases [12]. Furthermore this difference in tubule formation, Agnarsson et al [25] have reported more nuclear pleomorphism and higher mitotic rates in BRCA2 tumours than in sporadic tumours.

Hormone receptors and associated markers

Sporadic breast cancer tumours express a number of immunohistochemical markers that provide prognostic and predictive information about these tumours. In this sense, approximately 70% of breast carcinomas express the oestrogen receptor (ER) protein and 50% are progesterone receptor- (PR) positive. ER-positive tumours are more frequently well or moderately differentiated, p53- and HER2-negative and have a lower proliferation rate and better overall survival, but do not have a lower metastatic potential. ER-positive and PR-positive tumours respond to anti-oestrogen treatment in approximately 75% of the cases. The response is 34% when the tumour is ER-positive and PR-negative, and 45% in the case of ER-negative and PR-positive tumours. Only a small proportion of ER-negative and PR-negative (<10%) respond to anti-oestrogens, perhaps reflecting false negative ER measurement [30].

Many authors have emphasized the high frequency of ER-negativity in BRCA1 tumours, [10, 18, 28, 29, 3134]. Between 73% and 90% [28, 29, 31] of BRCA1 carcinomas have been reported to be ER-negative in different series. Although it has been suggested that this relationship might be explained by the higher grade of tumours and younger age of patients, BRCA1 tumours are more likely ER-negative than sporadic ones, when tumours from patients at the same age are compared. In addition, the likelihood of ER-negativity is 4.8 times higher in BRCA1 grade 3 tumours when they are compared with grade 3 sporadic cases [18]. In this sense, for example, Lakhani et al [10] reported that 90% of BRCA1-related breast cancers were ER-negative compared with 35% in controls.

PR expression in BRCA1 tumours is also lower than in sporadic tumours. In the study of Lakhani et al [10] 79% of BRCA1 tumours were PR-negative compared with 41% in sporadic tumours. The same proportion has been observed in other publications [28, 29, 31].

In contrast to BRCA1 tumours, those arising in BRCA2 mutation carriers do not differ from controls with regard to ER and PR expression. Thus, ER expression has been reported in around 65% of BRCA2 tumours [10, 31, 32]. Between 40% and 60% of BRCA2 carcinomas expressed PR [10, 31, 32].

Taking into account the differences in hormone receptor status between BRCA1 and BRCA2 tumours, it is not surprising that the expression of some markers is consistently associated with hormone receptors in breast cancer, such as Bcl-2 and cyclin D1, and differ between both genotypes. Bcl-2 is a protein that inhibits programmed cell death (apoptosis). This protein is detected in 50-90% of IDC of the breast [35, 36]. Bcl-2 expression is associated with favourable clinicopathological features [37]. Thus, it is more frequently present in well-differentiated ER-positive breast carcinomas. Bcl-2-positive p53-negative tumours have a better response to hormonal therapy than bcl-2-negative p53-positive ones [35]. Bcl-2 has a low rate of expression in BRCA1-associated tumours (31%) with respect to sporadic carcinomas [28, 38], but there are no statistically significant differences between BRCA2 and sporadic tumours [38].

Cyclin D1 plays an important role in cell cycle progression during G1 phase. Cyclin D1 is considered a potential oncogene and is amplified in about 15% of IDCs [39]. Cyclin D1 is known to be upregulated by oestrogen and progesterone and to be downregulated by anti-oestrogens [40]. The cyclin D1 protein is detected in 50% of cases [41] and its expression is associated with low histological grade, ER positivity and good prognosis. Several series have detected lower cyclin D1 expression levels in BRCA1 (5% and 33%) than in sporadic tumours [31, 33, 42]. Cyclin D1 expression in BRCA2 tumours has been found at an intermediate level between BRCA1 and sporadic tumours. Osin et al [33] reported that 27% of BRCA2 tumours expressed cyclin D1 compared with 5% in BRCA1 tumours, and 35% in sporadic tumours. In the same way, Armes et al [31] has shown cyclin D1 positivity in 55% of BRCA2, 33% of BRCA1 and 100% of sporadic tumours.

p53 expression and gene mutations

Around 15% to 30% of sporadic breast carcinomas have mutations in the tumour suppressor gene p53. Most of them are missense mutations that result in the accumulation of stable p53 protein that can be visualized by immunohistochemistry. However, 20% of the mutations do not yield a stable protein and it is not detected by immunostaining. For this reason, a stronger association between clinicopathological variables and p53 mutations, detected by DNA sequencing has been shown rather than with p53 immunohistochemistry detection in breast cancer.

Several studies have demonstrated a high incidence of p53 immunostaining in BRCA1 when compared to sporadic cases. p53 over-expression has been detected in 45% to 77% of BRCA1-associated tumours [10, 28, 29, 43, 44]. The results in BRCA2 tumours are inconclusive [10]. Some studies have found p53 over-expression in around 50% of BRCA2 carcinomas, whereas in other series the percentage was lower than 20% [28].

The importance of p53 inactivation in BRCA-associated tumours is also supported by genetic studies demonstrating a higher frequency and unusual location of p53 mutations in this group when compared with sporadic cases [4345]. In a review of reported cases by Chappuis et al [22] around 40% of BRCA1 and 30% of BRCA2 carcinomas had p53 mutations whereas it was found in only about 20% of sporadic controls. When comparing the spectrum of p53 mutations in BRCA1/2 patients with those reported in the IARC p53 mutation database, Greenblatt et al [46] found that they differed significantly both in distribution and in base changes. Mutations at A:T bp were common in BRCA1/2-associated tumours. Changes were frequent at p53 codons that are not mutation hotspots and were located at the opposite side of the p53 DNA-binding site.

HER2 expression and gene amplification

HER2 is the human epidermal growth factor receptor-2 that encodes a transmembrane glycoprotein with intrinsic tyrosine kinase activity. HER2 gene is located in chromosome 17q12 and has been associated with poor outcome and poor response to anti-oestrogens, but a better response to anthracyclines. Over-expression of HER-2 is identified in 10% to 34% of primary breast tumours [47, 48] and is attributable to gene amplification in approximately 90% of cases [49, 50]. The humanized anti-HER-2 monoclonal antibody, Herceptin, has been shown to be effective in 20% of patients with HER-2 amplification.

Data regarding HER2 expression in BRCA-associated tumours vary among series, probably indicating technical issues. For example, Armes et al [31] have shown no differences in strong expression of HER-2 between BRCA2 and sporadic breast tumours, 44% and 45% respectively, but they did not find HER-2 expression in any BRCA1 tumours. However, subsequent studies have observed HER2 over-expression in up to 3% in both BRCA1 and BRCA2 tumours [10, 28, 51]. With regard to the HER-2 amplification, only two studies have analyzed gene status by FISH in hereditary BRCA1-associated carcinomas [28, 52], and only one in BRCA2-associated carcinomas. According to immunohistochemistry data, high level of HER-2 amplification (>2.4 copies) has not been reported in BRCA1 and BRCA2 tumours.

BRCA1 carcinomas and the basal cell phenotype

Sorlie et al [53, 54] using cDNA microarrays classified breast tumours into clinically relevant subgroups. They defined different subtypes of breast tumours, including two non-overlapping groups of ER-negative carcinomas: the basal like and the ERBB2 over-expressing groups. The basal cell phenotype is characterized by the expression of markers that are usually expressed by the basal or myoepithelial cells of the normal breast. The group of myoepithelial/basal markers include the basal epithelial keratins (CK) 5/6, 14, and 17, muscle-specific actin, P-cadherin, S100, CD10, caldesmon, calponin, EGFR etc. [5557]. The existence of a group of breast carcinomas with a basal cell phenotype has been confirmed by other groups using DNA microarray and immunohistochemical approaches [5860]. Some of these studies have also indicated the poor prognosis of this group of neoplasias [17, 55, 56].

Recently Sorlie et al [53] reanalyzing cDNA microarray data from van't Veer et al [61], that included 18 BRCA1 and 2 BRCA2 carcinomas, observed that 80% of BRCA1 carcinomas had a basal epithelial type gene expression profile. Subsequently, Foulkes et al and Palacios et al have found a high prevalence of tumours with a basal phenotype among BRCA1 mutation carriers. For example, Palacios et al [60] reported that CK 5/6 was expressed in 50% of BRCA1 tumours but in less than 10% of BRCA2 cases and sporadic controls. In addition this phenotype is characterized by ER and HER2 negativity and the expression of specific cell cycle markers, such as over-expression of cyclin E and down-regulation of p27. Interestingly, a high proportion (60%) of BRCA1 carcinomas have been reported to over-express EGFR [10, 16, 57, 60, 62].

Histopathology of familial non-BRCA1/2 tumours

There are two studies that have defined the histological characteristics of these neoplasias [28, 63]. This group of tumours represents 67% of familial breast cancer when families with only female breast cancer and 4-5 affected members are considered [6]. In the Spanish population, when considering families with at least three cases of female breast cancer and one of them <50 years, the percentage of cases non-attributable to BRCA1/2 mutation was 75% [8].

IDC is the most frequent histological type in familial non-BRCA1/2: 77% of the cases according to Lakhani et al [63] and 78% in the series by Palacios et al [28]. An excess of lobular carcinomas was found in familial non-BRCA1/2 (15%) when compared with BRCA1 (3%), BRCA2 (9%) and sporadic cases (10%). The difference was only significant with respect to BRCA1 tumours [63].

Breast cancers from familial non-BRCA1/2 patients were of lesser histological grade than BRCA1/2-associated tumours [28, 63]. Thus, grade 1 tumours accounted for 27% to 50% in total. In addition, non-BRCA1/2 tumours showed more tubule formation, a lower mitotic index and lesser pleomorphism than BRCA1/2-associated carcinomas [28, 63].

In the only immunohistochemical study in familial non-BRCA1/2 [28], these tumours were more frequently ER- (75%), PR- (67%), and BCL2-positive (55%), but p53-negative (3.7%); these figures clearly differed from BRCA1 tumours, but no significant differences were found with respect to BRCA2 carcinomas. A low incidence of HER2 expression and amplification (4%) was found in non-BRCA1/2 carcinomas [28].

Pathology findings in prophylactic mastectomy specimens from BRCA1/2 mutation carriers

The natural history of hereditary breast cancer from morphologically normal epithelium to invasive disease is not well understood. The incidence of in situ lesions associated with invasive carcinomas is poorly described in most publications. Overall, an in situ component is less common around IDC in BRCA1 mutation carriers than in controls [12]. In addition, the incidence of in situ lesions in the absence of an invasive component in familial breast cancer is not established.

The study of prophylactic mastectomy specimens from BRCA1/2 mutation carriers has been proposed as a means to better understand the different stages of breast cancer development in these patients. However, the lack of data available is not conclusive due to the low number of case studies and the difficulty in selecting appropriate controls which is confounded by difficulties in recognizing precursor lesions.

Hoogerbrugge et al have reported a study with 67 patients at high risk of breast cancer, 44 of them with BRCA mutation, who underwent unilateral or bilateral prophylactic mastectomy. One or more different types of high-risk precursor lesions were present in 57% of the women: 37% had atypical lobular hyperplasia (ALH), 39% atypical ductal hyperplasia (ADH), 25% lobular carcinoma in situ (LCIS), and 15% ductal carcinoma in situ (DCIS). A 4-mm invasive ductal carcinoma was found in one woman with ductal carcinoma in situ. Prior to mastectomy, palpation or mammography was performed and none of these lesions were detected. These high risk lesions were more frequent in the group of women aged 40 years and older (73% vs. 43%) and less frequent in BRCA mutation carriers (43%) and women who had undergone bilateral oophorectomy before prophylactic mastectomy (20%) [64].

In another study, Kauff et al [65] compared prophylactic mastectomy specimens from 24 women with BRCA mutations and 48 controls extracted from an autopsy registry, concluding that BRCA mutation carriers have a higher incidence of high risk lesions including DCIS, LCIS, ADH or ALH (46%) compared to control cases (6%).

Adem et al [66] compared prophylactic mastectomy from patients with a family history of breast cancer including 28 BRCA1/2 mutation carriers, 117 women without BRCA1/2 mutation, 12 unclassified mutation variant carriers, and 283 sporadic control cases. They found similar prevalence of DCIS in all groups 53%, 60%, 56%, 55%, respectively. However, in BRCA1/2 mutation carriers, the prevalence of proliferative fibrocystic changes was lower (7%) compared with 25% in sporadic cases. In contrast, the proportion of invasive carcinomas was higher in mutation and unclassified variant alteration carriers compared with the control group and the mutation negative group, suggesting a faster progression of precursor lesions in mutation carriers.

Genetic testing and therapeutic implications

Available data concerning morphological and immunohistochemical characteristics of hereditary breast cancer demonstrated major differences between genotypes. The most important differences were found between tumours in BRCA1 carriers and all other categories. These histopathological features in conjunction with clinical data can be used to predict BRCA1 status and to a lesser extent BRCA2 and non-BRCA1/2 status and this could have implications for the process of mutation screening.

In this sense, it has been suggested that the immunohistochemical analysis of ER, together with age and histological grade, provides a new powerful predictor of BRCA1 mutation status. It has been estimated that the probability of a woman with familial breast cancer diagnosed before 35 years to be a BRCA1 mutation carrier is 25% if the tumour is grade 3 and ER-negative but the probability is only 5% if the tumour is ER-positive [10]. Taken into account the high proportion of BRCA1 carcinomas that express basal markers (i.e. CK 5/6), it is likely that the addition of such markers in the pathological study will improve our ability to predict the BRCA1 genotype.

Alternatively, if we have a tumour which is high grade (grade 2-3), ER-, and PR-positive and CK5/6-negative in a family with more than six female breast cancers in the first and second generations, early-onset prostate cancer or male breast cancer, it is likely we are dealing with a BRCA2 tumour.

Finally, it is very rare to find a BRCA1 or BRCA2 mutation when we are confronted with a low grade tumour (grade 1) with high tubule formation, ER- and PR-positive and p53-negative and a very low proliferation index. The probability of being a non-BRCA1/2 tumour increases if there are between three and six female breast cancer cases in the family, without ovarian cancer or early onset prostate cancer. In such a situation, probably it would be sufficient to study only the more recurrent mutations in each population in order to reduce the possibility of leaving atypical positive cases without diagnosis.

From a therapeutic point of view, adjuvant hormone therapy is not indicated in most BRCA1 tumours, since they are ER- and PR-negative, but can be used in most BRCA2 and non-BRCA1/2 carcinomas. The use of hormone therapy as chemopreventive drugs in BRCA1 patients is debatable [18, 32, 6770]. Taking into account the low incidence of HER2 amplification/over-expression in most hereditary breast cancer, these tumours are not good candidates for treatment with Herceptin. However, the high incidence of EGFR recently reported in BRCA1 tumour [62] opens the possibility that these women can be treated with specific compounds, such as tyrosine kinase inhibitors or anti-EGFR monoclonal antibodies.

Table 1 Morphological and immunohistochemical profiles associated with hereditary breast cancer tumours


  1. 1.

    Miki Y, Swensen J, Shattuck-Eidens D, Futreal PA, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett LM, Ding W, Bell R, Rosenthal J, Hussey C, Trant T, Mc Clure M, Frye C, Hattier T, Phelps R, Haugen-Strano A, Katcher H, Yakumo K, Gholami Z, Saffer D, Stone S, Bayer S, Wray C, Bogden R, Dayananth P, Ward J, Tonin P, Narod S, Bristow PK, Norris FH, Helvering L, Morrison P, Rosteck P, Lai M, Barret JC, Lewis C, Neuhausen S, Cannon-Albrigh LA, Goldgar D, Wiseman R, Kamb A, Skolnick MH: A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science 1994, 266: 66–71. 10.1126/science.7545954

    Article  CAS  PubMed  Google Scholar 

  2. 2.

    Wooster R, Bignell G, Lancaster J, Swift S, Seal S, Mangion J, Collins N, Gregory S, Gumbs C, Micklem G: Identification of the breast cancer susceptibility gene BRCA2. Nature 1995, 378: 789–792. 10.1038/378789a0

    Article  CAS  PubMed  Google Scholar 

  3. 3.

    Easton DF, Bishop DT, Ford D, Crockford GP: Genetic linkage analysis in familial breast and ovarian cancer: results from 214 families. The Breast Cancer Linkage Consortium. Am J Hum Genet 52: 678–701.

  4. 4.

    Stratton MR, Ford D, Neuhasen S, Seal S, Wooster R, Friedman LS, King MC, Egilsson V, Devilee P, McManus R, Daly PA, Smyth E, Ponder BA, Peto J, Cannon-Albright LA, Easton DF, Goldgar DE: Familial male breast cancer is not linked to the BRCA1 locus on chromosome 17q. Nat Genet 1994, 7: 103–107. 10.1038/ng0594-103

    Article  CAS  PubMed  Google Scholar 

  5. 5.

    Szabo CI, King MC: Population genetics of BRCA1 and BRCA2. Am J Hum Genet 1997, 60: 1013–1020.

    PubMed Central  CAS  PubMed  Google Scholar 

  6. 6.

    Ford D, Easton DF, Stratton M, Narod S, Goldgar D, Devilee P, Bishop DT, Weber B, Lenoir G, Chang-Claude J, Sobol H, Teare MD, Struewing J, Arason A, Scherneck S, Peto J, Rebbeck TR, Tonin P, Neuhausen S, Barkardottir R, Eyfjord J, Lynch H, Ponder BA, Gayther SA, Birch JM, Lindblom A, Stoppa-Lyonnet D, Bignon Y, Borg A, Hamann U, Haites N, Scott RJ, Maugard CM, Vasen H, Seitz S, Cannon-Albright LA, Schofield A, Zelada-Hedman M, the Breast Cancer Linkage Consortium: Genetic heterogeneity and penetrance analysis of the BRCA1 and BRCA2 genes in breast cancer families. Am J Hum Genet 1998, 62: 676–689. 10.1086/301749

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  7. 7.

    Shih HA, Couch FJ, Nathanson KL, Blackwood MA, Rebbeck TR, Armstrong KA, Calzone K, Stopfer J, Seal S, Stratton MR, Weber BL: BRCA1 and BRCA2 mutation frequency in women evaluated in a breast cancer risk evaluation clinic. J Clin Oncol 2002, 20: 994–999. 10.1200/JCO.20.4.994

    Article  CAS  PubMed  Google Scholar 

  8. 8.

    Osorio A, Barroso A, Martinez B, Cebrian A, San Roman JM, Lobo F, Robledo M, Benitez J: Molecular analysis of the BRCA1 and BRCA2 genes in 32 breast and/or ovarian cancer Spanish families. Br J Cancer 2000, 82: 1266–1270. 10.1054/bjoc.1999.1089

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  9. 9.

    de la Hoya M, Osorio A, Godino J, Sulleiro S, Tosar A, Perez-Segura P, Fernandez C, Rodriguez R, Diaz-Rubio E, Benitez J, Devilee P, Caldes T: Association between BRCA1 and BRCA2 mutations and cancer phenotype in Spanish breast/ovarian cancer families: implications for genetic testing. Int J Cancer 2002, 97: 466–471. 10.1002/ijc.1627

    Article  CAS  PubMed  Google Scholar 

  10. 10.

    Lakhani SR, Vijver MJ, Jacquemier J, Anderson TJ, Osin PP, McGuffog L, Easton DF: The pathology of familial breast cancer: predictive value of immunohistochemical markers estrogen receptor, progesterone receptor, HER-2, and p53 in patients with mutations in BRCA1 and BRCA2. J Clin Oncol 2002, 20: 2310–2318. 10.1200/JCO.2002.09.023

    Article  CAS  PubMed  Google Scholar 

  11. 11.

    Lakhani SR, Jacquemier J, Sloane JP, Gusterson BA, Anderson TJ, Vijver MJ, Farid LM, Venter D, Antoniou A, Storfer-Isser A, Smyth E, Steel CM, Haites N, Scott RJ, Goldgar D, Neuhausen S, Daly PA, Ormiston W, McManus R, Scherneck S, Ponder BA, Ford D, Peto J, Stoppa-Lyonnet D, Bignon YJ, Struewing JP, Spurr NK, Bishop DT, Klijn JG, Devilee P, Cornelisse CJ, Lasset C, Lenoir G, Barkardottir RB, Egilsson V, Hamann U, Chang-Claude J, Sobol H, Weber B, Stratton MR, Easton DF: Multifactorial analysis of differences between sporadic breast cancers and cancers involving BRCA1 and BRCA2 mutations. J Natl Cancer Inst 1998, 90: 1138–1145. 10.1093/jnci/90.15.1138

    Article  CAS  PubMed  Google Scholar 

  12. 12.

    Lakhani SR, Easton DF, Stratton MR, Consortium tBCL: Pathology of familial breast cancer: differences between breast cancers in carriers of BRCA1 or BRCA2 mutations and sporadic cases. Breast Cancer Linkage Consortium. Lancet 1997, 349: 1505–1510.

    Google Scholar 

  13. 13.

    Armes JE, Egan AJ, Southey MC, Dite GS, McCredie MR, Giles GG, Hopper JL, Venter DJ: The histologic phenotypes of breast carcinoma occurring before age 40 years in women with and without BRCA1 or BRCA2 germline mutations: a population-based study. Cancer 1998, 83: 2335–2345. 10.1002/(SICI)1097-0142(19981201)83:11<2335::AID-CNCR13>3.0.CO;2-N

    Article  CAS  PubMed  Google Scholar 

  14. 14.

    Lakhani SR: The pathology of familial breast cancer: morphological aspects. Breast Cancer Res 1999, 1: 31–35. 10.1186/bcr10

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  15. 15.

    Phillips KA: Immunophenotypic and pathologic differences between BRCA1 and BRCA2 hereditary breast cancers. J Clin Oncol 2000, 18: 107S-112S.

    CAS  PubMed  Google Scholar 

  16. 16.

    Foulkes WD, Stefansson IM, Chappuis PO, Begin LR, Goffin JR, Wong N, Trudel M, Akslen LA: Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer. J Natl Cancer Inst 2003, 95: 1482–1485.

    Article  CAS  PubMed  Google Scholar 

  17. 17.

    Foulkes WD, Brunet JS, Stefansson IM, Straume O, Chappuis PO, Begin LR, Hamel N, Goffin JR, Wong N, Trudel M, Kapusta L, Porter P, Akslen LA: The prognostic implication of the basal-like (cyclin E high/p27 low/p53+/glomeruloid-microvascular-proliferation+phenotype of BRCA1-related breast cancer. Cancer Res 2004, 64: 830–835. 10.1158/0008-5472.CAN-03-2970

    Article  CAS  PubMed  Google Scholar 

  18. 18.

    Foulkes WD, Metcalfe K, Sun P, Hanna WM, Lynch HT, Ghadirian P, Tung N, Olopade OI, Weber BL, McLennan J, Olivotto IA, Begin LR, Narod SA: Estrogen receptor status in BRCA1- and BRCA2-related breast cancer: the influence of age, grade, and histological type. Clin Cancer Res 2004, 10: 2029–2034. 10.1158/1078-0432.CCR-03-1061

    Article  CAS  PubMed  Google Scholar 

  19. 19.

    Ridolfi RL, Rosen PP, Port A, Kinne D, Mike V: Medullary carcinoma of the breast: a clinicopathologic study with 10-year follow-up. Cancer 1977, 40: 1365–1385. 10.1002/1097-0142(197710)40:4<1365::AID-CNCR2820400402>3.0.CO;2-N

    Article  CAS  PubMed  Google Scholar 

  20. 20.

    Rubens JR, Lewandrowski KB, Kopans DB, Koerner FC, Hall DA, McCarthy KA: Medullary carcinoma of the breast. Overdiagnosis of a prognostically favorable neoplasm. Arch Surg 1990, 125: 601–604.

    Article  CAS  PubMed  Google Scholar 

  21. 21.

    Rapin V, Contesso G, Mouriesse H, Bertin F, Lacombe MJ, Piekarski JD, Travagli JP, Gadenne C, Friedman S: Medullary breast carcinoma. A reevaluation of 95 cases of breast cancer with inflammatory stroma. Cancer 1988, 61: 2503–2510. 10.1002/1097-0142(19880615)61:12<2503::AID-CNCR2820611219>3.0.CO;2-3

    Article  CAS  PubMed  Google Scholar 

  22. 22.

    Chappuis PO, Nethercot V, Foulkes WD: Clinico-pathological characteristics of BRCA1- and BRCA2-related breast cancer. Semin Surg Oncol 2000, 18: 287–295. 10.1002/(SICI)1098-2388(200006)18:4<287::AID-SSU3>3.0.CO;2-5

    Article  CAS  PubMed  Google Scholar 

  23. 23.

    Marcus JN, Watson P, Page DL, Narod SA, Lenoir GM, Tonin P, Linder-Stephenson L, Salerno G, Conway TA, Lynch HT: Hereditary breast cancer: pathobiology, prognosis, and BRCA1 and BRCA2 gene linkage. Cancer 1996, 77: 697–709. 10.1002/(SICI)1097-0142(19960215)77:4<697::AID-CNCR16>3.0.CO;2-W

    Article  CAS  PubMed  Google Scholar 

  24. 24.

    Marcus JN, Watson P, Page DL, Narod SA, Tonin P, Lenoir GM, Serova O, Lynch HT: BRCA2 hereditary breast cancer pathophenotype. Breast Cancer Res Treat 1997, 44: 275–277. 10.1023/A:1005830230664

    Article  CAS  PubMed  Google Scholar 

  25. 25.

    Agnarsson BA, Jonasson JG, Bjornsdottir IB, Barkardottir RB, Egilsson V, Sigurdsson H: Inherited BRCA2 mutation associated with high grade breast cancer. Breast Cancer Res Treat 1998, 47: 121–127. 10.1023/A:1005853022804

    Article  CAS  PubMed  Google Scholar 

  26. 26.

    Garne JP, Aspegren K, Linell F, Rank F, Ranstam J: Primary prognostic factors in invasive breast cancer with special reference to ductal carcinoma and histologic malignancy grade. Cancer 1994, 73: 1438–1448. 10.1002/1097-0142(19940301)73:5<1438::AID-CNCR2820730519>3.0.CO;2-Y

    Article  CAS  PubMed  Google Scholar 

  27. 27.

    Henson DE, Ries L, Freedman LS, Carriaga M: Relationship among outcome, stage of disease, and histologic grade for 22,616 cases of breast cancer. The basis for a prognostic index. Cancer 1991, 68: 2142–2149. 10.1002/1097-0142(19911115)68:10<2142::AID-CNCR2820681010>3.0.CO;2-D

    Article  CAS  PubMed  Google Scholar 

  28. 28.

    Palacios J, Honrado E, Osorio A, Cazorla A, Sarrio D, Barroso A, Rodriguez S, Cigudosa JC, Diez O, Alonso C, Lerma E, Sanchez L, Rivas C, Benitez J: Immunohistochemical characteristics defined by tissue microarray of hereditary breast cancer not attributable to BRCA1 or BRCA2 mutations: differences from breast carcinomas arising in BRCA1 and BRCA2 mutation carriers. Clin Cancer Res 2003, 9: 3606–3614.

    CAS  PubMed  Google Scholar 

  29. 29.

    Lynch BJ, Holden JA, Buys SS, Neuhausen SL, Gaffney DK: Pathobiologic characteristics of hereditary breast cancer. Hum Pathol 1998, 29: 1140–1144. 10.1016/S0046-8177(98)90427-0

    Article  CAS  PubMed  Google Scholar 

  30. 30.

    Clarke R, Liu MC, Bouker KB, Gu Z, Lee RY, Zhu Y, Skaar TC, Gomez B, O'Brien K, Wang Y, Hilakivi-Clarke LA: Antiestrogen resistance in breast cancer and the role of estrogen receptor signaling. Oncogene 2003, 22: 7316–7339. 10.1038/sj.onc.1206937

    Article  CAS  PubMed  Google Scholar 

  31. 31.

    Armes JE, Trute L, White D, Southey MC, Hammet F, Tesoriero A, Hutchins AM, Dite GS, McCredie MR, Giles GG, Hopper JL, Venter DJ: Distinct molecular pathogeneses of early-onset breast cancers in BRCA1 and BRCA2 mutation carriers: a population-based study. Cancer Res 1999, 59: 2011–2017.

    CAS  PubMed  Google Scholar 

  32. 32.

    Robson ME, Chappuis PO, Satagopan J, Wong N, Boyd J, Goffin JR, Hudis C, Roberge D, Norton L, Begin LR, Offit K, Foulkes WD: A combined analysis of outcome following breast cancer: differences in survival based on BRCA1/BRCA2 mutation status and administration of adjuvant treatment. Breast Cancer Res 2004, 6: R8-R17. 10.1186/bcr658

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  33. 33.

    Osin P, Gusterson BA, Philp E, Waller J, Bartek J, Peto J, Crook T: Predicted anti-oestrogen resistance in BRCA-associated familial breast cancers. Eur J Cancer 1998, 34: 1683–1686. 10.1016/S0959-8049(98)00248-2

    Article  CAS  PubMed  Google Scholar 

  34. 34.

    Osin PP, Lakhani SR: The pathology of familial breast cancer: immunohistochemistry and molecular analysis. Breast Cancer Res 1999, 1: 36–40. 10.1186/bcr11

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  35. 35.

    Hurlimann J, Larrinaga B, Vala DL: bcl-2 protein in invasive ductal breast carcinomas. Virchows Arch 1995, 426: 163–168. 10.1007/BF00192638

    Article  CAS  PubMed  Google Scholar 

  36. 36.

    Baccouche S, Daoud J, Frikha M, Mokdad-Gargouri R, Gargouri A, Jlidi R: Immunohistochemical status of p53, MDM2, bcl2, bax, and ER in invasive ductal breast carcinoma in Tunisian patients. Ann N Y Acad Sci 2003, 1010: 752–763. 10.1196/annals.1299.136

    Article  CAS  PubMed  Google Scholar 

  37. 37.

    Joensuu H, Pylkkanen L, Toikkanen S: Bcl-2 protein expression and long-term survival in breast cancer. Am J Pathol 1994, 145: 1191–1198.

    PubMed Central  CAS  PubMed  Google Scholar 

  38. 38.

    Freneaux P, Stoppa-Lyonnet D, Mouret E, Kambouchner M, Nicolas A, Zafrani B, Vincent-Salomon A, Fourquet A, Magdelenat H, Sastre-Garau X: Low expression of bcl-2 in Brca1-associated breast cancers. Br J Cancer 2000, 83: 1318–1322. 10.1054/bjoc.2000.1438

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  39. 39.

    Frierson HF Jr, Gaffey MJ, Zukerberg LR, Arnold A, Williams ME: Immunohistochemical detection and gene amplification of cyclin D1 in mammary infiltrating ductal carcinoma. Mod Pathol 1996, 9: 725–730.

    PubMed  Google Scholar 

  40. 40.

    Gillett CE, Lee AH, Millis RR, Barnes DM: Cyclin D1 and associated proteins in mammary ductal carcinoma in situ and atypical ductal hyperplasia. J Pathol 1998, 184: 396–400. 10.1002/(SICI)1096-9896(199804)184:4<396::AID-PATH1259>3.0.CO;2-G

    Article  CAS  PubMed  Google Scholar 

  41. 41.

    Gillett C, Smith P, Gregory W, Richards M, Millis R, Peters G, Barnes D: Cyclin D1 and prognosis in human breast cancer. Int J Cancer 1996, 69: 92–99. 10.1002/(SICI)1097-0215(19960422)69:2<92::AID-IJC4>3.0.CO;2-Q

    Article  CAS  PubMed  Google Scholar 

  42. 42.

    Loden M, Stighall M, Nielsen NH, Roos G, Emdin SO, Ostlund H, Landberg G: The cyclin D1 high and cyclin E high subgroups of breast cancer: separate pathways in tumorigenesis based on pattern of genetic aberrations and inactivation of the pRb node. Oncogene 2002, 21: 4680–4690. 10.1038/sj.onc.1205578

    Article  CAS  PubMed  Google Scholar 

  43. 43.

    Crook T, Brooks LA, Crossland S, Osin P, Barker KT, Waller J, Philp E, Smith PD, Yulug I, Peto J, Parker G, Allday MJ, Crompton MR, Gusterson BA: p53 mutation with frequent novel condons but not a mutator phenotype in BRCA1- and BRCA2-associated breast tumours. Oncogene 1998, 17: 1681–1689. 10.1038/sj.onc.1202106

    Article  CAS  PubMed  Google Scholar 

  44. 44.

    Phillips KA, Nichol K, Ozcelik H, Knight J, Done SJ, Goodwin PJ, Andrulis IL: Frequency of p53 mutations in breast carcinomas from Ashkenazi Jewish carriers of BRCA1 mutations. J Natl Cancer Inst 1999, 91: 469–473. 10.1093/jnci/91.5.469

    Article  CAS  PubMed  Google Scholar 

  45. 45.

    Goffin JR, Chappuis PO, Begin LR, Wong N, Brunet JS, Hamel N, Paradis AJ, Boyd J, Foulkes WD: Impact of germline BRCA1 mutations and overexpression of p53 on prognosis and response to treatment following breast carcinoma: 10-year follow up data. Cancer 2003, 97: 527–536. 10.1002/cncr.11080

    Article  CAS  PubMed  Google Scholar 

  46. 46.

    Greenblatt MS, Chappuis PO, Bond JP, Hamel N, Foulkes WD: TP53 mutations in breast cancer associated with BRCA1 or BRCA2 germ-line mutations: distinctive spectrum and structural distribution. Cancer Res 2001, 61: 4092–4097.

    CAS  PubMed  Google Scholar 

  47. 47.

    Ross JS, Fletcher JA: HER-2/neu (c-erb-B2) gene and protein in breast cancer. Am J Clin Pathol 1999, 112: S53–67.

    CAS  PubMed  Google Scholar 

  48. 48.

    Pauletti G, Godolphin W, Press MF, Slamon DJ: Detection and quantitation of HER-2/neu gene amplification in human breast cancer archival material using fluorescence in situ hybridization. Oncogene 1996, 13: 63–72.

    CAS  PubMed  Google Scholar 

  49. 49.

    Kallioniemi OP, Kallioniemi A, Kurisu W, Thor A, Chen LC, Smith HS, Waldman FM, Pinkel D, Gray JW: ERBB2 amplification in breast cancer analyzed by fluorescence in situ hybridization. Proc Natl Acad Sci USA 1992, 89: 5321–5325. 10.1073/pnas.89.12.5321

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  50. 50.

    Tsuda H, Akiyama F, Terasaki H, Hasegawa T, Kurosumi M, Shimadzu M, Yamamori S, Sakamoto G: Detection of HER-2/neu (c-erb B-2) DNA amplification in primary breast carcinoma. Interobserver reproducibility and correlation with immunohistochemical HER-2 overexpression. Cancer 2001, 92: 2965–2974. 10.1002/1097-0142(20011215)92:12<2965::AID-CNCR10156>3.0.CO;2-A

    Article  CAS  PubMed  Google Scholar 

  51. 51.

    Johannsson OT, Idvall I, Anderson C, Borg A, Barkardottir RB, Egilsson V, Olsson H: Tumour biological features of BRCA1-induced breast and ovarian cancer. Eur J Cancer 1997, 33: 362–371. 10.1016/S0959-8049(97)89007-7

    Article  CAS  PubMed  Google Scholar 

  52. 52.

    Grushko TA, Blackwood MA, Schumm PL, Hagos FG, Adeyanju MO, Feldman MD, Sanders MO, Weber BL, Olopade OI: Molecular-cytogenetic analysis of HER-2/neu gene in BRCA1-associated breast cancers. Cancer Res 2002, 62: 1481–1488.

    CAS  PubMed  Google Scholar 

  53. 53.

    Sorlie T, Tibshirani R, Parker J, Hastie T, Marron JS, Nobel A, Deng S, Johnsen H, Pesich R, Geisler S, Demeter J, Perou CM, Lonning PE, Brown PO, Borresen-Dale AL, Botstein D: Repeated observation of breast tumor subtypes in independent gene expression data sets. Proc Natl Acad Sci USA 2003.

    Google Scholar 

  54. 54.

    Sorlie T, Perou CM, Tibshirani R, Aas T, Geisler S, Johnsen H, Hastie T, Eisen MB, Rijn M, Jeffrey SS, Thorsen T, Quist H, Matese JC, Brown PO, Botstein D, Eystein Lonning P, Borresen-Dale AL: Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proc Natl Acad Sci USA 2001, 98: 10869–10874. 10.1073/pnas.191367098

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  55. 55.

    Simpson PT, Gale T, Reis-Filho JS, Jones C, Parry S, Steele D, Cossu A, Budroni M, Palmieri G, Lakhani SR: Distribution and significance of 14–3-3sigma, a novel myoepithelial marker, in normal, benign, and malignant breast tissue. J Pathol 2004, 202: 274–285. 10.1002/path.1530

    Article  CAS  PubMed  Google Scholar 

  56. 56.

    Jones C, Mackay A, Grigoriadis A, Cossu A, Reis-Filho JS, Fulford L, Dexter T, Davies S, Bulmer K, Ford E, Parry S, Budroni M, Palmieri G, Neville AM, O'Hare MJ, Lakhani SR: Expression profiling of purified normal human luminal and myoepithelial breast cells: identification of novel prognostic markers for breast cancer. Cancer Res 2004, 64: 3037–3045. 10.1158/0008-5472.CAN-03-2028

    Article  CAS  PubMed  Google Scholar 

  57. 57.

    Kovacs A, Walker RA: P-cadherin as a marker in the differential diagnosis of breast lesions. J Clin Pathol 2003, 56: 139–141. 10.1136/jcp.56.2.139

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  58. 58.

    Callagy G, Cattaneo E, Daigo Y, Happerfield L, Bobrow LG, Pharoah PD, Caldas C: Molecular classification of breast carcinomas using tissue microarrays. Diagn Mol Pathol 2003, 12: 27–34. 10.1097/00019606-200303000-00004

    Article  CAS  PubMed  Google Scholar 

  59. 59.

    Rijn M, Perou CM, Tibshirani R, Haas P, Kallioniemi O, Kononen J, Torhorst J, Sauter G, Zuber M, Kochli OR, Mross F, Dieterich H, Seitz R, Ross D, Botstein D, Brown P: Expression of cytokeratins 17 and 5 identifies a group of breast carcinomas with poor clinical outcome. Am J Pathol 2002, 161: 1991–1996.

    PubMed Central  Article  PubMed  Google Scholar 

  60. 60.

    Palacios J, Honrado E, Osorio A, Diez O, Rivas C, Benitez J: Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer. J Natl Cancer Inst 2004, 96: 712–714. author reply 714.

    Article  PubMed  Google Scholar 

  61. 61.

    van't Veer LJ, Dai H, Vijver MJ, He YD, Hart AA, Mao M, Peterse HL, Kooy K, Marton MJ, Witteveen AT, Schreiber GJ, Kerkhoven RM, Roberts C, Linsley PS, Bernards R, Friend SH: Gene expression profiling predicts clinical outcome of breast cancer. Nature 2002, 415: 530–536. 10.1038/415530a

    Article  Google Scholar 

  62. 62.

    Groep P, Bouter A, Zanden R, Menko FH, Buerger H, Verheijen RH, Wall E, van Diest PJ: Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer.

  63. 63.

    Lakhani SR, Gusterson BA, Jacquemier J, Sloane JP, Anderson TJ, Vijver MJ, Venter D, Freeman A, Antoniou A, McGuffog L, Smyth E, Steel CM, Haites N, Scott RJ, Goldgar D, Neuhausen S, Daly PA, Ormiston W, McManus R, Scherneck S, Ponder BA, Futreal PA, Peto J, Stoppa-Lyonnet D, Bignon YJ, Stratton MR: The pathology of familial breast cancer: histological features of cancers in families not attributable to mutations in BRCA1 or BRCA2. Clin Cancer Res 2000, 6: 782–789.

    CAS  PubMed  Google Scholar 

  64. 64.

    Hoogerbrugge N, Bult P, de Widt-Levert LM, Beex LV, Kiemeney LA, Ligtenberg MJ, Massuger LF, Boetes C, Manders P, Brunner HG: High prevalence of premalignant lesions in prophylactically removed breasts from women at hereditary risk for breast cancer. J Clin Oncol 2003, 21: 41–45. 10.1200/JCO.2003.02.137

    Article  CAS  PubMed  Google Scholar 

  65. 65.

    Kauff ND, Brogi E, Scheuer L, Pathak DR, Borgen PI, Hudis CA, Offit K, Robson ME: Epithelial lesions in prophylactic mastectomy specimens from women with BRCA mutations. Cancer 2003, 97: 1601–1608. 10.1002/cncr.11225

    Article  PubMed  Google Scholar 

  66. 66.

    Adem C, Reynolds C, Soderberg CL, Slezak JM, McDonnell SK, Sebo TJ, Schaid DJ, Myers JL, Sellers TA, Hartmann LC, Jenkins RB: Pathologic characteristics of breast parenchyma in patients with hereditary breast carcinoma, including BRCA1 and BRCA2 mutation carriers. Cancer 2003, 97: 1–11. 10.1002/cncr.11048

    Article  PubMed  Google Scholar 

  67. 67.

    King MC, Wieand S, Hale K, Lee M, Walsh T, Owens K, Tait J, Ford L, Dunn BK, Costantino J, Wickerham L, Wolmark N, Fisher B: Tamoxifen and breast cancer incidence among women with inherited mutations in BRCA1 and BRCA2: National Surgical Adjuvant Breast and Bowel Project (NSABP-P1) Breast Cancer Prevention Trial. Jama 2001, 286: 2251–2256. 10.1001/jama.286.18.2251

    Article  CAS  PubMed  Google Scholar 

  68. 68.

    Clarke RB, Howell A, Potten CS, Anderson E: Dissociation between steroid receptor expression and cell proliferation in the human breast. Cancer Res 1997, 57: 4987–4991.

    CAS  PubMed  Google Scholar 

  69. 69.

    Rebbeck TR, Levin AM, Eisen A, Snyder C, Watson P, Cannon-Albright L, Isaacs C, Olopade O, Garber JE, Godwin AK, Daly MB, Narod SA, Neuhausen SL, Lynch HT, Weber BL: Breast cancer risk after bilateral prophylactic oophorectomy in BRCA1 mutation carriers. J Natl Cancer Inst 1999, 91: 1475–1479. 10.1093/jnci/91.17.1475

    Article  CAS  PubMed  Google Scholar 

  70. 70.

    Narod SA, Brunet JS, Ghadirian P, Robson M, Heimdal K, Neuhausen SL, Stoppa-Lyonnet D, Lerman C, Pasini B, de los Rios P, Weber B, Lynch H: Tamoxifen and risk of contralateral breast cancer in BRCA1 and BRCA2 mutation carriers. Hereditary Breast Cancer Clinical Study Group. Lancet 356: 1876–1881. 10.1016/S0140-6736(00)03258-X

Download references

Author information



Corresponding author

Correspondence to José Palacios.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Honrado, E., Benítez, J. & Palacios, J. The Pathology of Hereditary Breast Cancer. Hered Cancer Clin Pract 2, 131 (2004).

Download citation


  • BRCA1
  • BRCA2
  • non-BRCA1/2
  • histopathology