Fig. 2From: Prevalence of RECQL germline variants in Pakistani early-onset and familial breast cancer patientsRT-PCR analysis of the RECQL c.868-2A > G splice-site variant. a Photograph of an ethidium bromide-stained gel of the RECQL transcripts. Lane 1, DNA 100 bp marker; lanes 2 and 3, c.868-2A > G carriers; lanes 4 and 5, non-carriers (family member, control); lane 6, no template control; lane 7, gDNA wild type control; lane 8, Lambda DNA/HindIII marker. Product sizes: gDNA = 2260 bp; cDNA = 364 bp. Sequencing profiles of forward strand using PCR product from the cDNA of: b non-carrier (control), c-d c.868-2A > G carriers, e non-carrier (family member)Back to article page