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Table 1 Sequences of forward and reverse primers used for the detection of expression of BRCA1 isoforms by RT-PCR

From: When is a mutation not a mutation: the case of the c.594-2A>C splice variant in a woman harbouring another BRCA1 mutation in trans

Isoform

Forward sequence

Reverse sequence

Wildtype BRCA1 (does not cover truncation in exon 11)

5′-GCAACTTATTGCAGTGTGGG-3′

5′-ACAAGCAGCCTTTTTTGCAG-3′

Exon 10 deleted (Δ10)

5′- GCAACTTATTGCAGCTGCTTG-3′

5′- AGCTGCACGCTTCTCAGTG-3′

Exon 9 and 10 deleted (Δ9/10)

5′- GTCTGTCTACATTGAATTGGCTG-3′

5′- AGCTGCACGCTTCTCAGTG-3′

Exon 11q deleted (Δ11q) [12]

5′-CCAACTCTCTAACCTTGGAACTGTG-3′

5′-GATGACCTTTCCACTCCTGGTTC-3′