Fig. 1
From: Next-generation sequencing for genetic testing of familial colorectal cancer syndromes
a Mutations detected at next generation sequencing with the Ion Ampliseq custom panel. b A representative example of Sanger sequencing validation of a mutation identified using next generation sequencing (sample HNPCC6). On the left is the representation of the results of next-generation sequencing where the reads are aligned to the reference genome as provided by the Integrative Genomics Viewer (IGV v.2.1, Broad Institute) software. On the right is the representation of the results of Sanger sequencing