Figure 4
From: Identification of a new BRCA2 large genomic deletion associated with high risk male breast cancer
Mutation effect at transcript level. Agarose gel (1,5%) showing no amplification of proband’s samples using a forward primer in exon 13 and a reverse primer in exon 15 of BRCA2. Total cDNA from two independent RNA extractions from MRC5-V1 cells was used as a positive control for fragment amplification. A total of 70 ng from cDNA was used in each reaction. The length of the fragment expected is 530 bp.