Table 1 Real-Time PCR Assay-by-Design Primers and Probes for MUTYH Y165C, G382D and S501F
From: Genetic variants in MUTYH are not associated with endometrial cancer risk
SNP | Forward | Reverse | Wild Type Probe | Mutant Probe |
|---|---|---|---|---|
MUTYH S501 (C>T) | CAGCCTTCCAAAAGGTTCCCA | GCTGTGTGCATCAGTGGAGAT | VIC-CACGGAG AGGACAC | FAM-CACGGAA AGGACAC |
MUTYH Y165C (A>G) | CCACAGGAAGGTGAATCAACTCT | CCTTACCTTCCGAGCTCCCT | VIC-CTGGGCTA CTATTCT | FAM-GGGCTG CTATTCT |
MUTYH G382D (G>A) | GACCCCTGCCTGGCT | GACGGGAACTCCCACAGT | VIC-CCTCTCAGG TCTGCTG | FAM-CCTCTCAGA TCTGCTG |