Figure 1

Detection of CHEK2 1100 delC mutation by real-time allele-specific PCR. When normal DNA is subjected to PCR, the amplification driven by the wild-type specific primer significantly exceeds that initiated by mutation-specific oligonucleotide (left). In the case of CHEK2 1100 delC heterozygosity, both wild-type and mutation-specific curves cross their threshold of detection at approximately the same cycle (right).