Table 1 DHPLC conditions for analyses of exons 7 and 13 of the MYH gene
From: MYH Gene Status in Polish FAP Patients without APC Gene Mutations
Exon 7 (Y165C) | ||||
|---|---|---|---|---|
Time (min) | % Buffer A | % Buffer B | Oven temp. | |
loading | 0.0 | 55.9 | 44.1 | 62.8°C |
start gradient | 0.5 | 50.9 | 49.1 | |
stop gradient | 5.0 | 41.9 | 58.1 | |
Exon 13 (G382D) | ||||
Time (min) | %A | AB | ||
loading | 0.0 | 53.9 | 46.1 | 65.6°C |
start gradient | 0.5 | 48.5 | 51.5 | |
stop gradient | 5.0 | 39.9 | 60.1 |