Table 1 Primers used for the analysis of exon 15 of the APC gene
From: Germline Missense Changes in the APC Gene and Their Relationship to Disease
Segment | Sequence | Oven Temperature (°C) |
|---|---|---|
FAPex15.1For | gttactgcatacacattgtgac | 50,59 |
FAPex15.1Rev | gctttttgtttcctaacatgaag | Â |
FAPex15.2For | agtacaaggatgccaatattatg | 50,59 |
FAPex15.2Rev | acttctatctttttcagaacgag | Â |
FAPex15.3For | atttgaatactacagtgttaccc | 50,54,60 |
FAPex15.3Rev | cttgtattctaatttggcataagg | Â |
FAPex15.4For | ctgcccatacacattcaaacac | 50,55,57 |
FAPex15.4Rev | tgtttgggtcttgcccatctt | Â |
FAPex15.5For | agtcttaaatattcagatgagcag | 50,52 |
FAPex15.5Rev | gtttctcttcattatattttatgcta | Â |
FAPex15.6For | aagcctaccaattatagtgaacg | 50,59 |
FAPex15.6Rev | agctgatgacaaagatgataatg | Â |
FAPex15.7For | aagaaacaatacagacttattgtg | 50,54,59 |
FAPex15.7Rev | atgagtggggtctcctgaac | Â |
FAPex15.8For | atctccctccaaaagtggtgc | 50,60 |
FAPex15.8Rev | tccatctggagtactttccgtg | Â |
FAPex15.9aFor | agtaaatgctgcagttcagagg | 50,58 |
FAPex15.9aRev | catcatcatctgaatcatcta | Â |
FAPex15.9bFor | accaagagaaagaggcag | 50,54,59 |
FAPex15.9bRev | ccgtggcatatcatccccc | Â |
FAPex15.10For | gcccagactgcttcaaaattacc | 50, 61 |
FAPex15.10Rev | gagcctcatctgtacttctgc | Â |
FAPex15.11For | ccctccaaatgagttagctgc | 50,54,60 |
FAPex15.11Rev | ttgtggtataggttttactggtg | Â |
FAPex15.12For | acccaacaaaaatcagttagatg | 50,57 |
FAPex15.12Rev | gtggctggtaactttagcctc | Â |
FAPex15.13For | atgatgttgacctttccaggg | 50,58 |
FAPex15.13Rev | gctcagtctctttgataggttc | Â |
FAPex15.14For | ctgagttctctcagtgacattgac | 50,58 |
FAPex15.14Rev | gttctgaatctggtctctg | Â |
FAPex15.15For | tatgggtggcatattaggtg | 50,59 |
FAPex15.15Rev | cctttactttcagattctatc | Â |
FAPex15.16For | aggcccacgaattctaaaacc | 50,54,58 |
FAPex15.16Rev | cctggcaacagggcttaattc | Â |
FAPex15.17For | gaaggtcaaacagccacc | 50,59 |
FAPex15.17Rev | ggcattcttggataaacctg | Â |
FAPex15.18For | catctccaggtagacagatgag | 50,59 |
FAPex15.18Rev | cttaaaactggagtttgtgcctg | Â |
FAPex15.19For | ctccatcatctagaccagc | 50,61 |
FAPex15.19Rev | cactggattctgatgaagc | Â |
FAPex15.20For | tggagaagaactggaagttc | 50,58 |
FAPex15.20Rev | gggagatcttccagatctagg | Â |
FAPex15.21For | acagaggatgtttgggtgag | 50,60 |
FAPex15.21Rev | gcttgagctgctagaactg | Â |
FAPex15.22For | cctgtatcagagactaatg | 50,55,60 |
FAPex15.22Rev | caaaatgtctatatagcagttg | Â |