Pedigree and LOH Testing in Tumors of c.3113G > A Mutation Family. (A) Pedigree of the family carrying the c.3113G > A mutation. Circles = females; Squares = males. Filled symbols = affected with cancer. Slashed symbols = deceased. +/+ = wild-type; +/- = heterozygous carrier; d. = death, numbers denote age, the arrow denotes the proband. (B) Loss of heterozygosity analysis of the proband’s breast cancer shows both alleles are still present in the tumor. (C) Hematoxylin-eosin stain of the tumor tissue analyzed in panel B.