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Table 5 Nested-PCR amplification of oocytes–second stage of amplification-recurrence of the results for oocytes obtained from the same amplification conditions for each mutation

From: Comparison of whole genome amplification and nested-PCR methods for preimplantation genetic diagnosis for BRCA1 gene mutation on unfertilized oocytes–a pilot study

Mutation Amount of amplified gene fragments in one sample Number of samples [n] Average Recurrence
5382insC 4/5 3 0,47 47%
2/5
1/5
185delAG 2/5 3 0,67 67%
3/5
5/5
C61G 5/5 2 2 100%
  10/10   1