Comparison of whole genome amplification and nested-PCR methods for preimplantation genetic diagnosis for BRCA1 gene mutation on unfertilized oocytes–a pilot study

Table 3 The sequences of the primers used in the nested-PCR reaction

Mutation	Sequence of the primers	mp	Product length
5382insC	F: 5’ AGTGATCTGCCTGCCTCAGT 3’	64,1°C	315 pz
5382insC	R: 5’ CCATCTCTGCAAAGGGGAGT 3’	65,4°C	315 pz
185delAG	F: 5’ TTGGAGAAAGCTAAGGCTACCA 3’	65,4°C	685 pz
185delAG	R: 5’ CCCAGTGCAGAACCAATCA 3’	64,9°C	685 pz
C61G	F: 5’ TTGCTTATGCAGCATCCAAA 3’	64,2°C	633 pz
	R: 5’ GCACTCCAGCCTCAGTGAC 3’	63,9°C

ISSN: 1897-4287