Comparison of whole genome amplification and nested-PCR methods for preimplantation genetic diagnosis for BRCA1 gene mutation on unfertilized oocytes–a pilot study

Table 2 The sequences of the primers used in the RFLP reaction

Mutation	Sequence of the primers	mp	Product length
C61G	F: 5’ CTC TTA AGG GCA GTT GTG AG 3’	59,1°C	158 pz
	R: 5’ ATG GTT TTA TAG GAA CGC TAT G 3’	58,6°C	118 pz

ISSN: 1897-4287