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Table 2 The sequences of the primers used in the RFLP reaction

From: Comparison of whole genome amplification and nested-PCR methods for preimplantation genetic diagnosis for BRCA1 gene mutation on unfertilized oocytes–a pilot study

Mutation Sequence of the primers mp Product length
C61G F: 5’ CTC TTA AGG GCA GTT GTG AG 3’ 59,1°C 158 pz
  R: 5’ ATG GTT TTA TAG GAA CGC TAT G 3’ 58,6°C 118 pz