Comparison of whole genome amplification and nested-PCR methods for preimplantation genetic diagnosis for BRCA1 gene mutation on unfertilized oocytes–a pilot study

Table 1 The sequences of the primers used in the ASA-PCR reaction

Mutation	Sequence of the primers	mp	Product length
5382insC	N: 5’ AGAGAATCCCAGGACA 3’	68,2°C	168 pz
	M: 5’ AGAGAACTCCCAGGAC 3’	81,8°C
	R: 5’ ATATGACGTGTCTGCTCCAC 3’	64,1°C
185delAG	N: 5’ GCTGACTTACCAGATGGGACTCTC 3’	66,1°C	335 pz
185delAG	M: 5’ CCCAAATTAATACACTCTTGTCGTGACTTACCAGATGGGACAGTA 3’	78,4°C	335 pz
	R: 5’ GGT TGG CAG CAA TAT GTG AA 3’	63,4°C

ISSN: 1897-4287