Novel germline CDK4 mutations in patients with head and neck cancer

Table 1 List of primers with annealing temperature and product size (bp)

Exon No	Primer sequence (Forward) F	Primer sequence (Reverse) R	Product size (bp)	Annealing temperature °C
1	TCACGTGCCCAGAACGTC	CTCATTCCTGGGAAGGGACT	266	56
2	AAGCGACTTTTGGTGATAGGA	TTACTCCCCACGCCCAAC	308	54
3	GAGAGGCCATGTTGGGTTAA	TCCACCTCTCAATGCCTACC	222	58
4	TCTGGGATTTCAGGTATGGTG	ATTGCTACGGGCAATCACTC	295	58
5	GTGTTTCATGGTAACCCATGG	TTTATGAACAAGCGATTTGGG	219	58
6	TCTTTGAAAATGACTGCTACCTT	CCTGGGTTCAGCAGAAAGAG	156	58
7	AGGACCCTCCTGACCAGAGT	CTTTCCCTGTGCCCACAG	205	60
8	TCATGGTTTTCTGACCTTTGC	GCCCTCTCAGTGTCCAGAAG	190	58

ISSN: 1897-4287