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Table 3 Mutations identified by DHPLC with the predicted and actual temperatures of detection and a summary of which mutations were identified by DGGE

From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Gene/Exon Mutation Description Fragment Size (bp) Predicted Temperature Actual Temperature Detected Detected by DGGE/DHPLC
hMLH1
1 G to A (116) splice site 200 62 60 y/y
1 A to G (1) 200 62 60 y/y
1 A to G (112) 200 62 60 y/y
4 C to T (350) 191 54,59 59 y/y
4 G to A (+1) 191 54,59 54 y/y
6 C to T (+51) 230 56 56 y/y
7 T to G (554) 205 55 58 y/y
8 A to G (655) 149 48,53,55,58 58 y/y
9 A to G (790) splice site 181 56 58 y/y
10 G to A (884) 213 58 58 n/y
13 G to A (+13) 209 59 57,58,59 y/y
13 G to T (1535) 209 59 58,59 y/y
15 G to A (1731) splice site 210 49,54 54 y/y
16 AA to GC (1852-3) 259 55,60 55,60 y/y
17 G to T (1959) 178 59 58,59 y/y
17 G to A (1989) splice site 178 59 57,58,59 y/y
19 A to G (2253) 266 59 56,57 y/y
19 G to A (2147) 266 59 58,59 y/y
hMSH2
1 C of G (+8) 300 60 64/67 y/y
2 G to A (304) 244 53,54 54 n/y
3 C to T (547) 359 48/53/58 58 n/y
3 A to T (488) 359 48/53/58 58 y/y
6 C to T (1009) 240 57 57 y/y
6 G to A (965) 240 57 57 y/y
7 A to T (+2) 326 51/56 51 n/y
7 C to T (+7) 326 51/56 51 n/y
7 C to T (1216) 326 51/56 57 n/y
7 A to T (-2) 326 51/56 51 n/y
9 G to C (-1) 183 55 55 y/y
10 A to G (+12) 249 50,55 50,52,53,55 y/y
10 C to A (1632) 249 50,55 50,52,53 y/y
11 T to C (1669) 198 2 52,53 y/y
11 A to g (1690) 198 55 55 y/y
12 G to C (1968) 319 52,57 56,57 y/y
13 A to G (2157) 284 54,59 54,56 y/y
13 T to C (+6) 284 54,59 54,56 y/y
Deletions
hMLH1
1 1 bp (9) 200 62 62 y/y
3 4 bp (+2, 208-9) 181 55 55 y/y
7 1 bp (588) 205 55 55 y/y
8 1 bp (672) 149 48,53,58 55 y/y
13 5 bp (1460-1464) 209 59 50,54,58,59 y/y
16 3 bp (1846-48) 259 55,60 53 y/y
17 2 bp (1986-1987) 178 59 57,58,59 y/y
19 1 bp (2114) 266 59 56 y/y
hMSH2
1 1 bp (163) 300 60 64,66,67 n/y
7 1 bp (1260) 326 51/56 57 n/y
9 1 bp (1408) 183 55 50,53,55 y/y
15 7 bp (2502-2508) 266 58 50,52,56 y/y
Insertions
hMLH1
12 4 bp (1164) 410 55,60 50,55,60 y/y
Inversions
hMSH2
8 23 bp (1338-1361) 212 53 53,50 y/y