A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Meldrum, Cliff J.; McPhillips, Mary; Crooks, Renee; Thomas, Lesley; Edkins, Ted; Creegan, Rohanna; Miller, Ewan; Agrez, Michael; Scott, Rodney J.

doi:10.1186/1897-4287-1-1-39

Hereditary Cancer in Clinical Practice

Table 2 Primers used for DHPLC analysis of hMLH1 and hMSH2

From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

hMLH1	Forward	Reverse
Exon 1	GAA CAT CTA GAC GTT TCC	GTT AAG TCG TAG CCC TTA
Exon 2	CAT TAG AGT AGT TGC AGA CTG	CGC ACA AAC ATC CTG CTA CT
Exon 3	GAG TAA CAT GAT TAT TTA CTC	AAC AGG AGG ATA TTT TAC ACA
Exon 4	CAG TTC AGA TAA GGT TTC C	TAT GAG TAA AAG AAG TCA GCA
Exon 5	CTC TCT ACT GGA TAT TAA TTT G	AAG CTT CAA CAA TTT ACT CTC C
Exon 6	CTA TCT TAA GAC CTC GCT	TAT GAG CAC TAG AAC ACA TT
Exon 7	TCT AGT GTG TGT TTT TGG CA	CAT GGC TGA GAC TGA AAC
Exon 8	AAT AAA TCC TTG TGT CTT CTG	TTT TTA TAT AGG TTA TCG ACA TA
Exon 9	TTA TGC TTC AGA ATC TCT TTT C	TGT TTC CTG TGA GTG GAT
Exon 10	TTT GTG TGA ATG TAC ACC TG	TCT GTT CCT TGT GAG TCT
Exon 11	CTA AGG TAA TTG TTC TCT CTT A	CAG AGA AGT AGC TGG ATG
Exon 12	TAA TAC AGA CTT TGC TAC CAG GA	GGT AGG CTG TAC TTT TCC CA
Exon 13	CTG CAC TTC CTT TTC TTC AT	CCT ATG CAT CCC AGG CA
Exon 14	AGG ATT CTA TTA CTT ACC TG	TCT GCT TGT TCA CAC ACT CA
Exon 15	CAA CTG GTT GTA TCT CAA G	TAC AAA TAA GAT ATT AGT GGA GA
Exon 16	CTT GGG AAT TCA GGC TTC	AGA AGT ATA AGA ATG GCT GTC A
Exon 17	CAG CAT TAT TTC TTG TTC CC	CAC ATG CAT GTA CCG AAA
Exon 18	ATT CGT ACC TAT TTT GAG GT	TTG TAT AGG CCT GTC CTA
Exon 19	AGC CAG GAC ACC AGT GTA	GAA CAC ATC CCA CAG TGC
hMSH2	Forward	Reverse
Exon 1	CTT CAA CCA GGA GGT GAG	AAA GGA GCC GCG CCA
Exon 2	CAG CTA ATA CAG TGC TTG	GTA AAT TAA AAA GGA AGA TAA TTA C
Exon 3	GCT TAT AAA ATT TTA AAG TAT GTT C	GCC TTT CCT AGG CCT GGA ATC TCC
Exon 4	TTC ATT TTT GCT TTT CTT ATT CC	ATA TGA CAG AAA TAT CCT TC
Exon 5	CAG TGG TAT AGA AAT CTT CG	CCA ATC AAC ATT TTT AAC CC
Exon 6	GTT TTC ACT AAT GAG CTT GCC	ATG TGG GTA ACT GCA GGT TAC
Exon 7	GAC TTA CGT GCT TAG TTG	GTA TAT ATT GTA TGA GTT GAA GG
Exon 8	TTG TAT TCT GTA AAA TGA GAT C	GCT TTT TAA AAA TAA CTA CTG C
Exon 9	GGA TTT TGT CAC TTT GTT CTG	ATT ATT CCA ACC TCC AAT GAC
Exon 10	GGT AGT AGG TAT TTA TGG AA	CAT TTA GGG AAT TAA TAA AGG G
Exon 11	CAC ATT GCT TCT AGT ACA C	CCA GGT GAC ATT CAG AAC
Exon 12	ATT CAG TAT TCC TGT GTA CA	CCA CAA AGC CCA AAA ACC A
Exon 13	CAA TCC ATT TAT TAG TAG CAG	TTC TAT CTT CAA GGG ACT AG
Exon 14	TAC CAC ATT TTA TGT GAT GGG	TAC CAA GTT CTG AAT TTA GAG
Exon 15	TTC TCA TGC TGT CCC CTC A	AGA GAA GCT AAG TTA AAC TAT G
Exon 16	TAA TTA CTC ATG GGA CAT TCA C	TAC CTT CAT TCC ATT ACT GG

These primers were based on Liu et al. [9], Wijnen et al. [24], Kolodner et al. [27], Liu et al. [28], with minor modification.

Back to article page

ISSN: 1897-4287

Contact us

General enquiries: journalsubmissions@springernature.com