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Table 1 Increasing urea gradients used for DGGE analysis of all exons comprising hMLH1 and hMSH2

From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Denaturant Gradient Gene/Exon
15-30% HMSH2 exon 16
20-50% HMSH2 exon 4
25-45% HMSH2 exons 2, 8, 12
30-40% HMSH2 exons 3, 5, 7, 11
30-45% HMSH2 exons 6, 9
30-50% HMSH2 exons 14, 15
35-45% HMSH2 exon 1, hMLH1 exons 3, 9, 15
38-48% hMLH1 exon 7
40-50% hMLH1 exons 4, 5, 6, 8, 10, 16, 17
40-60% hMLH1 exon 18
40-70% hMLH1 exon 11
42-52% hMLH1 exon 19
45-55% hMSH2 exon 13, hMLH1 exons 2, 12, 13, 14
50-70% hMSH2 exon 1, hMLH1 exon 1