Table 1 Increasing urea gradients used for DGGE analysis of all exons comprising hMLH1 and hMSH2
Denaturant Gradient | Gene/Exon |
|---|---|
15-30% | HMSH2 exon 16 |
20-50% | HMSH2 exon 4 |
25-45% | HMSH2 exons 2, 8, 12 |
30-40% | HMSH2 exons 3, 5, 7, 11 |
30-45% | HMSH2 exons 6, 9 |
30-50% | HMSH2 exons 14, 15 |
35-45% | HMSH2 exon 1, hMLH1 exons 3, 9, 15 |
38-48% | hMLH1 exon 7 |
40-50% | hMLH1 exons 4, 5, 6, 8, 10, 16, 17 |
40-60% | hMLH1 exon 18 |
40-70% | hMLH1 exon 11 |
42-52% | hMLH1 exon 19 |
45-55% | hMSH2 exon 13, hMLH1 exons 2, 12, 13, 14 |
50-70% | hMSH2 exon 1, hMLH1 exon 1 |