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Figure 4 | Hereditary Cancer in Clinical Practice

Figure 4

From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Figure 4

Predictive ability of DHPLC profiles. Identical profiles were observed at the predicted oven temperature for both a 1009 C>T substitution (A) and a 965 G to A substitution (B) in exon 6 of the hMSH2 gene. These profiles were different to those obtained for a 984 C to T substitution (C) and no substitutions (D) in the same fragment. PCR products (5 μl) were separated on a Helix DHPLC column under partially denaturing conditions using a flow rate of 0.45 ml/min and an increasing linear acetonitrile gradient.

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