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Figure 3 | Hereditary Cancer in Clinical Practice

Figure 3

From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Figure 3

Mutations undetected by DGGE that were detected by DHPLC. PCR products (5 μl) were separated on a Helix column at the indicated temperature and a 0.45 ml/min flow rate. A linear increasing acetonitrile gradient was used to elute the DNA from the column. Arrows indicate the presence of heteroduplex species on DHPLC traces for (A) hMLH1 exon 6,544 +51 T to C; (B) hMSH2 exon 13,1558 +6T to C and (C) hMLH1 exon 10,884 G to A.

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