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Figure 2 | Hereditary Cancer in Clinical Practice

Figure 2

From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGE

Figure 2

Mutations and polymorphisms in the same PCR fragment distinguished by their DHPLC elution profile. PCR products (5 μl) were separated on a Helix DHPLC column under partially denaturing conditions at the indicated temperature using a flow rate of 0.45 ml/min and an increasing linear acetonitrile gradient. Arrows on DHPLC traces indicate the presence of heteroduplex species. The polymorphism is a 655G to A substitution and the mutation a 672delT, both in exon 8 of the hMLH1 gene. (A) sample homozygous for the polymorphism and heterozygous for the mutation. (B) sample heterozygous for both the polymorphism and the mutation; (C) sample homozygous for both the polymorphism and mutation; (D) sample heterozygous for the polymorphism and homozygous for the mutation; (E) same as D separated at 58°C

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