The contribution of CHEK2 to the TP53-negative Li-Fraumeni phenotype
© Ruijs et al; licensee BioMed Central Ltd. 2009
Received: 14 November 2008
Accepted: 17 February 2009
Published: 17 February 2009
CHEK2 has previously been excluded as a major cause of Li-Fraumeni syndrome (LFS). One particular CHEK2 germline mutation, c.1100delC, has been shown to be associated with elevated breast cancer risk. The prevalence of CHEK2*1100delC differs between populations and has been found to be relatively high in the Netherlands. The question remains nevertheless whether CHEK2 germline mutations contribute to the Li-Fraumeni phenotype.
We have screened 65 Dutch TP53-negative LFS/LFL candidate patients for CHEK2 germline mutations to determine their contribution to the LFS/LFL phenotype.
We identified six index patients with a CHEK2 sequence variant, four with the c.1100delC variant and two sequence variants of unknown significance, p.Phe328Ser and c.1096-?_1629+?del.
Our data show that CHEK2 is not a major LFS susceptibility gene in the Dutch population. However, CHEK2 might be a factor contributing to individual tumour development in TP53-negative cancer-prone families.
Li-Fraumeni syndrome (LFS) is a rare autosomal dominant cancer syndrome predisposing for bone and soft tissue sarcoma, breast cancer, brain tumour, adrenocortical carcinoma and leukaemia . The classical LFS criteria are: a proband with sarcoma aged under 45 years and a first-degree relative with any cancer aged under 45 years, plus a first or second-degree relative in the same lineage with any cancer under the age of 45 years or sarcoma at any age . In addition, Li-Fraumeni-like syndrome (LFL) criteria have been formulated as a proband with any childhood tumour or a sarcoma, brain tumour or adrenocortical tumour diagnosed under 45 years of age and a first or second-degree relative in the same lineage with a typical LFS tumour at any age, plus a first or second-degree relative in the same lineage younger than 60 years with any cancer . Less stringent LFL criteria were formulated by Eeles et al. as two first or second-degree relatives with typical LFS-extended tumours (classical LFS tumours plus melanoma, prostate cancer and pancreatic cancer) at any age . The Chompret criteria for TP53 germline mutation testing have been updated in 2008 as: (1) a proband with a tumour belonging to the LFS tumour spectrum (sarcomas, brain tumours, pre-menopausal breast cancer, adrenocortical carcinoma, leukaemia, lung bronchoalveolar cancer) cancer before 46 years of age and at least one first or second-degree relative with an LFS tumour before 56 years of age or multiple tumours; or (2) a proband with multiple tumours two of which belong to the narrow LFS tumour spectrum and the first of which occurred before 46 years of age; or (3) a patient with adrenocortical carcinoma or a patient with breast cancer before 36 years of age without BRCA mutation, irrespective of the family history .
In 1990 germline mutations in the TP53 gene were described in LFS . So far, 419 TP53-positive families have been reported (IARC mutation database, R13, November 2008 ). At present, in approximately 75% of LFS and 40% of LFL families, a germline TP53 mutation can be detected ; i.e. 25% to 60% of LFS/LFL families do not carry a detectable germline TP53 mutation, implying the existence of alternative LFS susceptibility genes.
CHEK2 is a cell cycle checkpoint kinase involved in DNA repair, cell death and cell cycle control by stabilizing the p53 protein . In 1999 Bell et al. first described the possible association of the CHEK2 gene with Li-Fraumeni syndrome . Subsequent studies have addressed the possible contribution of CHEK2 germline mutations to LFS and LFL syndrome, but could not confirm CHEK2 as a major gene involved in LFS [10–18].
In other studies, the specific CHEK2 c.1100delC frameshift mutation was shown to be associated with an elevated breast cancer risk [19–22] and it has been suggested that it contributes to a hereditary breast and colorectal cancer phenotype . The prevalence of this c.1100delC mutation seems to differ according to ethnic backgrounds and populations and is relatively high in the Netherlands [19, 24]. We have investigated the CHEK2 gene mutation status of 65 index patients from 65 Dutch LFS/LFL families and families suggestive of LFS who had tested negative for TP53 germline mutations, to determine the contribution of CHEK2 germline mutations to the phenotype in those families.
Number of TP53 negative families available for CHEK2 gene analysis divided into 3 groups: LFS, LFL, or LFS-suggestive family history (n = 65), including the cancer type in tested individuals.
Complete CHEK2 mutation analysis
(n = 34)
1100delC mutation analysis and DNA rearrangements
(n = 31)
Cancer type in tested individuals: B/S/other
-childhood onset sarcoma or brain tumour
-at least 2 primary tumours
-2 first degree relatives with cancer
-breast cancer before 30 years
DNA from peripheral blood lymphocytes was isolated according to standard procedures. Screening for TP53 germline mutations was performed by sequence analysis of all coding exons (2–11) including flanking intron-exon boundaries (details are available on request) and multiplex ligation-dependent probe amplification (MLPA)  (TP53 MLPA KIT, MRC Holland). In 34 TP53-negative LFS, LFL, or LFS-suggestive families all exons and flanking intron-exon boundaries of the CHEK2 gene were investigated using denaturing gradient gel electrophoresis (DGGE, see Table 1) . All possible candidate variants, identified as aberrant DGGE fragments, were confirmed by sequence analysis. To avoid amplification of pseudogenes, a long range PCR was performed first for exons 10 to 14, followed by a nested PCR. Data on exons 1–10 were obtained for all patients, on exons 11–14 for 29 of the 34 individuals. All 65 TP53-negative individuals were screened for the c.1100delC CHEK2 mutation and CHEK2 DNA rearrangements by multiplex ligation-dependent probe amplification (MLPA, see Table 1). Details are available on request (CHEK2 MLPA KIT, MRC Holland). Mutation analysis was performed using the following reference sequence: CHEK2 (AF086904.1, GI:3982839, http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=3982839).
Sequence variants were weighted according to their potential pathogenicity. Three silent sequence variants were seen and not further analysed: c.252A>G, p.Glu84Glu in exon 1, a previously reported silent polymorphism , found once, c.1566C>T, p.Pro522Pro and c.1608A>G, p.Pro536Pro, both in exon 14, found in five and seven families, respectively. For these three variants two splice site prediction programs were used, NetGene2 Server http://www.cbs.dtu.dk/services/NetGene2/ and BDGP Splice Site Prediction/Neural Network http://www.fruitfly.org/seq_tools/splice.html; no alternative splice sites were predicted.
When possible, the presence of a sequence variant detected in an index patient was investigated in other affected relatives. A control group of 150 anonymous Dutch (male and female) blood donors was analysed by DGGE to determine the prevalence of the sequence variants in a general population sample.
A chi-square test was used to determine the statistical significance of the proportion of CHEK2 mutation carriers in our study group compared to healthy controls.
Results and discussion
Sixty-five TP53-negative individuals from 65 families were screened for the CHEK2 1100delC germline mutation and DNA rearrangements. Thirty-four of these individuals were screened comprehensively by DGGE for CHEK2 mutations. Six index patients were found to carry a possibly pathogenic germline CHEK2 sequence variant.
Another sequence variant, c.983T>C, p.Phe328Ser in exon 8, localised in the kinase domain of the gene, was detected in a female patient who had developed a leiomyosarcoma at 2 years of age and a schwannoma at 27 years of age (Figure 1E). The family of the index patient fulfilled the LFL criteria (Eeles ). The parents of the index patient are both healthy and over 60 years of age. A maternal aunt died of breast cancer at 45 years of age and a sister of the index patient's maternal grandmother died of a brain tumour; no material was available for testing. Under the assumption that these two affected family members were carriers of the mutation, penetrance would be incomplete with non-penetrance exhibited by two older healthy obligate mutation carriers. The hypothesis that the exon 8 mutation caused the complete LFL phenotype in this family is unlikely although a de novo mutation, contributing to the disease in the index patient, cannot be excluded. The p.Phe328Ser missense mutation has not been described in the literature before and was not found in 150 healthy Dutch controls. The phenylalanine in this position is conserved in mice and frogs but not in either zebrafish or C. Elegans (Ensembl, v39-Jun 2006 ).
One CHEK2 DNA rearrangement was found, c.1096-?_1629+?del, a deletion of exons 10–14 of the gene. The family fulfilled the LFL criteria according to Birch and the index patient developed breast cancer at the age of 55 years. A deletion of this size in the kinase domain of the gene will probably abolish the kinase activity. This deletion has not been described in the literature before. A deletion of exon 9–10 predicting protein truncation at codon 381 was discovered as a founder mutation among patients of Czecholovakian ancestry with breast cancer . Unfortunately, no material from the daughter who developed a rhabdomyosarcoma at 22 years of age was available. Four sibs of the index patient were healthy; her father developed stomach cancer at 55 years of age.
Literature on CHEK2 analysis in LFS and LFS-related families
number of families tested: LFS/LFL/suggestive
total CHEK2 analysis
CHEK2 1100delC analysis + DNA rearrangements
Allinen et al. 
Bell et al. 
Bougeard et al. 
Lee et al. 
Siddiqui et al. 
Sodha et al. 
Vahteristo et al. 
Walsh et al. 
In our study six index patients were found to carry a CHEK2 sequence variant by screening 65 TP53-negative index patients, with no evidence that the sequence variants found caused the complete LFS phenotype in their families. Our data are in line with the hypothesis that the CHEK2 c.1100delC might be associated with an elevated breast cancer risk [19, 20], and possibly with a breast and colorectal cancer phenotype  or more generally a multi-organ cancer susceptibility . We propose that the germline CHEK2 sequence variants contribute to tumour development in the index patients. Without these tumours, the families would not have fulfilled the established LFS/LFL criteria and TP53 germline mutation testing would not have been indicated. In this way, the individual CHEK2 sequence variants may contribute to the Li-Fraumeni phenotype seen in these families.
Because only 75% of classical LFS families and 40% of LFL families have germline TP53 mutations, research groups have looked at candidate genes like Bcl10 , CDKN2 [34, 35], TP63 , PTEN [34, 36], CHEK1 [10, 14] and BAX ; no possible alternative LFS genes were found.
Two polymorphisms, p.Arg72Pro (TP53 gene) and SNP309 T>G (MDM2 gene), have been shown to have a modifying effect, resulting in an earlier age of onset of cancer in TP53 mutation carriers [38, 39]; there is even a synergistic effect when both polymorphisms are present. These are proposed examples of modifying factors or low penetrance gene mutations that play a role in age of onset and tumour clustering in cancer-prone families . In our present study group, we investigated the possible modifier effects of these polymorphisms but found no association with an earlier age of tumour onset  (p.Arg72Pro, data not shown). We did find a larger proportion of homozygotes for the G-allele of MDM2 SNP309 in our TP53-negative group, suggesting a modifier effect on the TP53 negative Li-Fraumeni phenotype.
Our data illustrate that CHEK2 is not a major LFS susceptibility gene in the Dutch population. The CHEK2 gene might be a factor contributing to individual tumour development in families that are subsequently recognised as having a Li-Fraumeni phenotype. Although many genes have been excluded as alternative LFS genes, many more modifiers or low penetrance susceptibility genes might occur in families showing a Li-Fraumeni phenotype.
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