Coding region | Forward sequence | Reverse sequence | Annealing temperature |
---|---|---|---|
Exon 1 | ggtgcctccggggctca | gaatgcgtccctcgcaagt | 70.5Ā°C |
Exon 2 | gagtcacccggttccatctacctt | ccagggacaccggcagc | 70.5Ā°C |
Exon 3 | tcttgtctttaatctgtccaatttcctaatctc | gcgcactaaaacaacagcga | 63.5Ā°C |
Exon 4 | tatccgtcttgaattgtcttatcttgttcctcatc | ccctcccagagaaacagagaactt | 63.5Ā°C |
Exon 5 | cagtgttgggatccttctttactaattc | cccggtgtcaacaagcttctaa | 63.5Ā°C |
Exon 6 | tttcttcctcatcagagctcaagt | tccaaagaacctaagagtctttctgagta | 69.0Ā°C |
Exon 7 | tcccaaagtgcagcttgtctaa | taatacacatttgtcctccacaccc | 60.5Ā°C |
Exon 8 | gtcctgacttggttgtgtcg | agacctttctttggaaaccctctaa | 69.0Ā°C |
Exon 9 | agtcttggtactttgtaaatgacacatct | agctgtgaggatgccagtt | 69.0Ā°C |
Exon 10 | aaatgtttcgttttgtttttaacttcattgt | ccagttgctgcaagtcagttga | 67.5Ā°C |
Exon 11 | tttcagctacatgttgtttgctggtcctatt | gctaggaggtcgaggcagcaaag | 60.5Ā°C |
Exon 12 | ttgccaagctgccacattt | gcatggcagttggagcaaag | 63.5Ā°C |
Exon 13 | cctcccctggtctcatcatttc | aagtcaaaggctgagtcacttgc | 67.5Ā°C |
Exon 14 | cttctttatctttggctctcaacactt | agagatcaccactgagctacc | 63.5Ā°C |
Exon 15 | cctactcttcattgtacttcaacct | tgagcttagagatgagccatgc | 63.5Ā°C |
Exon 16.1 | acaggtgtgcccttcctttcactaa | ccaccagcaacgtgatttctgcattt | 60.5Ā°C |