Figure 1From: A Comparison Between Denaturing Gradient Gel Electrophoresis and Denaturing High Performance Liquid Chromatography in Detecting Mutations in Genes Associated with Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and the Identification of 9 New Mutations Previously Unidentified by DGGEDHPLC and DGGE detection of base substitutions, deletions, and insertions in genes associated with HNPCC. PCR products (5 μl) were separated on a Helix DHPLC column under partially denaturing conditions (at the indicated temperatures) using a flow rate of 0.45 ml/min and an increasing linear acetonitrile gradient. Arrows on DHPLC traces indicate the presence of a heteroduplex species and asterisks on DGGE gels indicate the sample represented by DHPLC analysis. (A) 116G to A introducing a stop codon in exon 1 of hMLH1. (B) 1460 (del5) in exon 13 of hMLH1 (C) 1164 (ins4) in exon of 12 hMLH1Back to article page