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Figure 3 | Hereditary Cancer in Clinical Practice

Figure 3

From: Differential expression of PTEN gene correlates with phenotypic heterogeneity in three cases of patients showing clinical manifestations of PTEN hamartoma tumour syndrome

Figure 3

PTEN protein analysis. Representative image of three repeated experiments qualitatively similar. A) Western blot assay of PTEN performed on protein extracts from peripheral blood cells. The amounts of PTEN and β-actin were measured by Western blot. II-3, III-4, III-3, III-2, III-1: patients-numbering corresponds to that adopted in the shown pedigree. B) Histogram showing optical density of PTEN protein normalized versus actin protein: Density of the electrophoretic band was obtained with ImageJ software. Relative quantification of PTEN protein normalized versus actin protein was calculated using sample III-3 as calibrator. C) Western blot assay of PTEN performed on protein extracts from colon mucosa. N2: healthy colon mucosa and P2: amartomatous polyp of proband’s daughter described in case 2; N3: healthy colon mucosa and T3: colorectal carcinoma of proband 3; C: healthy colon mucosa. D) Histogram showing optical density of PTEN protein normalized versus actin protein: Density of the electrophoretic band was obtained with ImageJ software. Relative quantification of PTEN protein normalized versus actin protein was calculated using sample N-2 as calibrator.

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Hereditary Cancer in Clinical Practice

ISSN: 1897-4287